Receives a GenBank file as input and returns the entire biological sequence in FASTA file. Use this tool to remove non-sequence information from a GenBank file.

Receives an EMBL file as input and returns the entire biological sequence in FASTA file. Use this tool to remove non-sequence information from an EMBL file.

Converts a protein sequence in one-letter code to a sequence in three-letter code.

Converts a protein sequence in three-letter code to a sequence in one-letter code.

Receives a RNA or DNA sequence and returns its contents of nitrogen bases.

Receives a DNA sequence and returns its complementary sequence. In this program there is the possibility of obtain the reverse complementary sequence.

Receives a DNA sequence and performs the translation into amino acids, using the standard codon table. It is possible perform the translation for the three open reading frames (ORFs) in the direct strand (reading +1, +2 and +3) and in the reverse strand (reading -1, -2 and -3).

Receives a DNA sequence and returns its transcript sequence.

Receives a DNA sequence and returns the possible cleavage sites of most used restriction endonucleases, giving the name of the enzyme, the number and locations of the cuts along of the sequence.

Receives a DNA sequence and returns all the possible open reading frames (ORFs). In this tool, its possible obtain the three open reading frames in the direct strand (reading +1, +2 and +3) and in the reverse strand (reading -1, -2 and -3). The user can discriminate the minimum size of coding sequences to be screened.

Receives a DNA sequence and returns its forward and reverse terminal primers. It is possible also generate the internal primer from the input sequence. The user can determine the length of the primers, as well as adding endonuclease restriction sites.

Receives a protein sequence and returns the amount of each amino acid type. Moreover, returns the amount of hydrophobic, hydrophilic, positive, negative and aromatic amino acids residues and the amount of amino acids with hydroxyl group.

Receives an amino acid protein sequence and returns the position of its potential sites of proteolytic cleavage [⁴,⁵] of a user selected enzyme.

Receives an amino acid protein sequence and returns all theoric peptide fragments generated by proteolytic cleavage [⁴,⁵] of a user selected enzyme. The program also generates the monoisotopic and average mass, simulating monoprotonated fragments.

Receives a protein or peptide sequence and highlights a group of amino acids selected by user. The program contains pre-defined groups of amino acids to be marked: hydrophobic, hydrophilic, positive, negative and aromatic residues and amino acids with hydroxyl group. In addition, the user can choose the color to be used in the highlights and define its own group of amino acids.

Allows the user to create textual representations of peptide sequences. The program automatically calculates the average and monoisotopic mass, and returns its molecular formula.

The Easy Bioinformatics Analysis package has an intuitive and easy navigation. The programs can be accessed online by the main page accessible from http://www.iq.unesp.br/EXTENSAO/EBiAn/html/ebian.html (Figure 1). Moreover, it can also be used offline after downloaded it from the main page. The user with programming skills can access the source code and change it to create new programs or adapting them. The programs depend only of Web browsers and are compatible with Internet Explorer, Opera, Mozilla Firefox and Google Chrome, having better performance for the last two. In future versions of the package, the included programs will be improved and new programs may be included. Additional questions or comments regarding the Easy Bioinformatics Analysis package can be sent by e-mail to luiz_cbb@hotmail.com.