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Early use of granulocyte colony stimulating factor improves survival in a rabbit model of chronic myocardial ischemia.
MedLine Citation:
PMID:  23085036     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Granulocyte colony stimulating factor (G-CSF) improves the survival of animals with myocardial infarction by inducing bone marrow stem cell mobilization and homing to infarcted areas. However, its precise mechanisms and direct effects on the ischemic myocardium remain unclear. In this study we investigated the direct effects and mechanisms of G-CSF in a rabbit model of chronic myocardial ischemia. METHODS: Myocardial ischemia models were created by partial ligation of the left anterior descending coronary artery in Japanese white male rabbits. Rabbits were subcutaneously injected with 10μg/kg of G-CSF (G-CSF group) or saline (control group) for 6 days after myocardial ischemia. Direct effects of G-CSF were analyzed by immunohistochemistry and terminal dUTP nick end-labeling (TUNEL). RESULTS: Rabbits in the G-CSF group exhibited 75% survival compared to 40% in the control group (p<0.05). Immunohistochemistry of the ischemic myocardium showed increased homing of CD34+ cells on day 7 post-surgery and more vessels on day 28 post-surgery by anti-von Willebrand factor staining in the G-CSF group compared with the control group. Furthermore, an increased percentage of CD34+ cells were observed in peripheral blood and upregulation of vascular endothelial growth factor expression in ischemic tissue in the G-CSF group compared with the control group. TUNEL showed that the apoptotic index in the ischemic myocardium decreased in the G-CSF group compared with the control group on day 28 post-surgery. CONCLUSIONS: In addition to increasing stem cell mobilization and homing to ischemic myocardium, G-CSF treatment after myocardial ischemia improves survival by accelerating neovascularization and reducing apoptosis.
Authors:
Qingbin Zhao; Chaofeng Sun; Xin Xu; Juan Zhou; Yue Wu; Yuling Tian; Aiqun Ma; Zhiquan Liu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-18
Journal Detail:
Title:  Journal of cardiology     Volume:  -     ISSN:  1876-4738     ISO Abbreviation:  J Cardiol     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804703     Medline TA:  J Cardiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.
Affiliation:
Department of Cardiology, The First Affiliated Hospital of Medical College in Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China. Electronic address: zhaoqingbin05@163.com.
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