Document Detail

Early time course of recruitment of immune surveillance in human skin after chemical provocation.
MedLine Citation:
PMID:  8443959     Owner:  NLM     Status:  MEDLINE    
As part of the defence function of skin it seems probable that mechanisms exist for the rapid recruitment of immune surveillance to 'inspect' any foreign substance that penetrates the skin. In the present study, evidence of such mechanisms was sought by following the time course of early changes in distribution of immune cells, expression of cell adhesion molecules and cytokines after epicutaneous challenge with provoking chemicals to which subjects were known to be either specifically 'sensitive' or 'non-sensitive'; anthralin, an irritant chemical, was used as control. Fifty-seven individuals were studied and there were at least five biopsy samples at each time point. Regardless of whether individuals were sensitive or not, or of the type of chemical, dermal microvascular endothelial cells showed increased expression of the adhesion molecules ELAM-1 and VCAM-1 within 2 h, and ICAM-1 within 8 h. The intensity of immunohistochemical staining increased progressively up to 24 h. More vessels stained for ICAM-1 than for VCAM-1 or ELAM-1, implying that not every vessel expressed all three cell adhesion molecules. Another early change, observed 2 h after irritant challenge, was a significant increase in numbers of CD1a+ dendritic cells in the superficial dermis from a median of 3/high power field (hpf) to 9.5/hpf (P < 0.03). This was not observed with 'weak' provoking substances, such as nickel, but did occur with the potent provoking agent dinitrochlorobenzene (DNCB). Thus, as little as 2 h after contact with provoking chemicals, the skin activates cellular mechanisms to increase T cell infiltration for the presumed purpose of immune surveillance. These mechanisms are not dependent upon specific immune sensitivity and reflect a capacity of skin cells to respond to chemical provocation.
P S Friedmann; I Strickland; A A Memon; P M Johnson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  91     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-05     Completed Date:  1993-04-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  351-6     Citation Subset:  IM    
Dermatology Unit, Royal Liverpool University Hospital, UK.
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MeSH Terms
Cell Adhesion Molecules / analysis*
Cell Count
Cytokines / immunology
Dermatitis, Contact / immunology*,  pathology
Immunoenzyme Techniques
Immunologic Surveillance / immunology*
Skin / drug effects,  immunology*,  pathology
T-Lymphocytes / immunology*
Time Factors
Reg. No./Substance:
0/Allergens; 0/Cell Adhesion Molecules; 0/Cytokines

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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