| Early stimulation of RNA synthesis by erythropoietin in cultures of erythroid precursor cells. | |
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MedLine Citation:
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PMID: 4522297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of erythropoietin on cultured erythroid precursor cells from 13-day mouse-fetal livers was examined. Within 1 hr, erythropoietin causes a 2- to 3-fold stimulation of uridine incorporation into RNA by these cells. The types of RNA preferentially stimulated by erythropoietin during the first hour of exposure of the cells to the hormone include ribosomal RNAs and their precursors, as well as 4-5S RNA. No unique RNA species, not present in control cells, could be detected by sucrose gradient sedimentation or gel electrophoresis. Inhibition of protein synthesis for up to 1 hr does not abolish the stimulatory effect of erythropoietin on RNA synthesis, suggesting that the effect of the hormone on RNA synthesis is not mediated by a newly synthesized protein. |
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Authors:
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G M Maniatis; R A Rifkind; A Bank; P A Marks |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 70 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1973 Nov |
Date Detail:
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Created Date: 1974-05-22 Completed Date: 1974-05-22 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3189-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibiotics, Antineoplastic / pharmacology Carbon Radioisotopes Cells, Cultured Centrifugation, Density Gradient Cycloheximide / pharmacology Electrophoresis, Polyacrylamide Gel Erythropoietin / antagonists & inhibitors, pharmacology* Fetus Hematopoietic Stem Cells / drug effects, metabolism* Kinetics Leucine / metabolism Liver / metabolism Mice RNA / analysis, biosynthesis* RNA, Ribosomal / analysis, biosynthesis Stimulation, Chemical Tritium Uridine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antibiotics, Antineoplastic; 0/Carbon Radioisotopes; 0/RNA, Ribosomal; 10028-17-8/Tritium; 11096-26-7/Erythropoietin; 58-96-8/Uridine; 61-90-5/Leucine; 63231-63-0/RNA; 66-81-9/Cycloheximide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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