Document Detail


Early steps of sperm-egg interactions during mammalian fertilization.
MedLine Citation:
PMID:  8936405     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian eggs are surrounded by two egg coats: the cumulus oophorus and the zona pellucida, which is an extracellular matrix composed of sulfated glycoproteins. The first association of the spermatozoon with the zona pellucida occurs between the zona glycoprotein, ZP3 and sperm receptors, located at the sperm plasma membrane, such as the 95 kDa tyrosine kinase-protein. This association induces the acrosome reaction and exposes the proacrosin/acrosin system. Proacrosin transforms itself, by autoactivation, into the proteolytical active form: acrosin. This is a serine protease that has been shown to be involved in secondary binding of spermatozoa to the zona pellucida and in the penetration of mammalian spermatozoa through it. The zona pellucida is a specific and natural substrate for acrosin and its hydrolysis and fertilization can be inhibited by antiacrosin monoclonal antibodies. Moreover, in in vitro fertilization experiments, trypsin inhibitors significantly inhibits fertilization. The use of the silver-enhanced immunogold technique has allowed immunolocalization of the proacrosin/acrosin system in spermatozoa after the occurrence of the acrosome reaction. This system remains associated to the surface of the inner acrosomal membrane for several hours in human, rabbit and guinea-pig spermatozoa while in the hamster it is rapidly lost. In the hamster, the loss of acrosin parallels the capability of the sperm to cross the zona pellucida. Rabbit perivitelline spermatozoa can fertilize freshly ovulated rabbit eggs and retain acrosin in the equatorial and postacrosomal region. These spermatozoa also show digestion halos on gelatin plates that can be inhibited by trypsin inhibitors. This evidence strongly suggests the involvement of acrosin in sperm penetration through the mammalian zona. Recently it was shown, however, that acrosin would not be essential for fertilization. It is likely, then, that such an important phenomenon in the mammalian reproductive cycle would be ensured though several alternative mechanisms.
Authors:
C Barros; J A Crosby; R D Moreno
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Cell biology international     Volume:  20     ISSN:  1065-6995     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1997-01-02     Completed Date:  1997-01-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  33-9     Citation Subset:  IM    
Affiliation:
Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Santiago.
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MeSH Terms
Descriptor/Qualifier:
Acrosin / physiology
Acrosome / physiology
Animals
Egg Proteins / metabolism
Enzyme Precursors / physiology
Female
Guinea Pigs
Humans
Male
Membrane Glycoproteins / metabolism
Rabbits
Receptors, Cell Surface*
Sperm-Ovum Interactions / physiology*
Zona Pellucida / physiology
Chemical
Reg. No./Substance:
0/Egg Proteins; 0/Enzyme Precursors; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/zona pellucida glycoproteins; EC 3.4.21.-/proacrosin; EC 3.4.21.10/Acrosin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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