| Early steps in cell infection by parvoviruses: host-specific differences in cell receptor binding but similar endosomal trafficking. | |
| | |
MedLine Citation:
|
PMID: 19656887 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Canine parvovirus (CPV) and feline panleukopenia virus (FPV) are closely related parvoviruses that differ in their host ranges for cats and dogs. Both viruses bind their host transferrin receptor (TfR), enter cells by clathrin-mediated endocytosis, and traffic with that receptor through endosomal pathways. Infection by these viruses appears to be inefficient and slow, with low numbers of virions infecting the cell after a number of hours. Species-specific binding to TfR controls viral host range, and in this study FPV and strains of CPV differed in the levels of cell attachment, uptake, and infection in canine and feline cells. During infection, CPV particles initially bound and trafficked passively on the filopodia of canine cells while they bound to the cell body of feline cells. That binding was associated with the TfR as it was disrupted by anti-TfR antibodies. Capsids were taken up from the cell surface with different kinetics in canine and feline cells but, unlike transferrin, most did not recycle. Capsids labeled with fluorescent markers were seen in Rab5-, Rab7-, or Rab11-positive endosomal compartments within minutes of uptake, but reached the nucleus. Constitutively active or dominant negative Rab mutants changed the intracellular distribution of capsids and affected the infectivity of virus in cells. |
| | |
Authors:
|
Carole E Harbison; Sangbom Michael Lyi; Wendy S Weichert; Colin R Parrish |
Related Documents
:
|
12941917 - Heparan sulfates and coxsackievirus-adenovirus receptor: each one mediates coxsackievir... 8504147 - Glycosphingolipid compositions of human t-lymphotropic virus type i (htlv-i) and human ... 202227 - Studies on glycopeptides of herpes simplex virus infected cells. 9302217 - Development of an in vitro cloning method for cowdria ruminantium. 9281357 - O-glycosylation and cellular differentiation in a subpopulation of mucin-secreting ht-2... 19224207 - Export of functional streptomyces coelicolor alditol oxidase to the periplasm or cell s... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2009-08-05 |
Journal Detail:
|
Title: Journal of virology Volume: 83 ISSN: 1098-5514 ISO Abbreviation: J. Virol. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-24 Completed Date: 2009-10-16 Revised Date: 2010-09-27 |
Medline Journal Info:
|
Nlm Unique ID: 0113724 Medline TA: J Virol Country: United States |
Other Details:
|
Languages: eng Pagination: 10504-14 Citation Subset: IM |
Affiliation:
|
Baker Institute for Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Capsid Proteins / chemistry, metabolism Cats Cell Line Cells / virology* Dogs Endosomes / physiology Feline panleukopenia virus / metabolism, pathogenicity* Host-Pathogen Interactions* Parvovirus, Canine / metabolism, pathogenicity* Pseudopodia / virology* Receptors, Transferrin / metabolism* Receptors, Virus / metabolism Species Specificity rab GTP-Binding Proteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
|
AI 28385/AI/NIAID NIH HHS; AI 33468/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Capsid Proteins; 0/Receptors, Transferrin; 0/Receptors, Virus; EC 3.6.1.-/rab GTP-Binding Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cell entry of Borna disease virus follows a clathrin-mediated endocytosis pathway that requires Rab5...
Next Document: Analysis of human alphaherpesvirus microRNA expression in latently infected human trigeminal ganglia...