Document Detail

Early stages of implantation as revealed by an in vitro model.
MedLine Citation:
PMID:  20179188     Owner:  NLM     Status:  MEDLINE    
Our limited understanding of the processes underlying steroid hormonal control of human endometrial receptivity is largely due to the lack of a relevant model system. To overcome scarcity of material, we have developed a model in which mouse embryos attach to human Ishikawa cells, which express functional steroid hormone receptors. Blastocysts flushed from day 4 pregnant superovulated mice were transferred to confluent Ishikawa cell monolayers. After 48 h of co-culture, 85% of the blastocysts had attached loosely, but only 40% attached stably to the epithelial cell surface. In contrast, 95% of the embryos attached stably to tissue culture plastic. Thus, weak attachment of a majority of the embryos was followed by stronger adhesion of a smaller proportion. Seventeen percent of the transferred blastocysts modified the epithelial cell surface with loss of MUC1 at the attachment site, extending variably to adjacent epithelial cells. Initially, stable attachment occurred without disruption to the integrity of the epithelial monolayer, but at later stages after the embryo had spread laterally, displacement of subjacent cells was observed. A modest increase in stable attachment, but no changes to MUC1 clearance, was observed after assisted hatching. After 24 h priming of Ishikawa cells by 17beta-oestradiol (OE(2)) followed by 72-h incubation with medroxyprogesterone acetate and OE(2), stable attachment increased from 40 to 70%. Initial attachment is efficient either in the presence or in the absence of hormone; steroid treatment increased the incidence of stable attachment. Implantation failure is predicted to occur in this model when embryos fail to progress from initial to stable attachment.
H Singh; L Nardo; S J Kimber; J D Aplin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-23
Journal Detail:
Title:  Reproduction (Cambridge, England)     Volume:  139     ISSN:  1741-7899     ISO Abbreviation:  Reproduction     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100966036     Medline TA:  Reproduction     Country:  England    
Other Details:
Languages:  eng     Pagination:  905-14     Citation Subset:  IM    
Maternal and Fetal Health Research, Manchester Academic Health Sciences Centre, University of Manchester, Manchester, M13 9WL, UK.
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MeSH Terms
Antigens, CD164 / metabolism
Cell Line
Embryo Implantation / drug effects,  physiology*
Embryo, Mammalian / cytology,  physiology
Endometrium / cytology,  drug effects,  physiology*
Estradiol / pharmacology
Estrogens / pharmacology
Medroxyprogesterone Acetate / pharmacology
Models, Biological*
Mucin-1 / metabolism
Progestins / pharmacology
Superovulation / drug effects
Zona Pellucida / physiology
Reg. No./Substance:
0/Antigens, CD164; 0/Estrogens; 0/Mucin-1; 0/Progestins; 50-28-2/Estradiol; 71-58-9/Medroxyprogesterone Acetate

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