| Early response assessment in prostate carcinoma by ¹⁸F-fluorothymidine following anticancer therapy with docetaxel using preclinical tumour models. | |
| | |
MedLine Citation:
|
PMID: 20878403 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: The aim of the study was to assess the potential usefulness of 3-deoxy-3-(18)F-fluorothymidine (FLT) as a radiopharmaceutical for imaging the early therapeutic effects of docetaxel (DTX) on tumour proliferation in hormone-refractory prostate cancer (HRPC). METHODS: Cells of the androgen-independent human prostate tumour cell line, 22Rv1, were implanted in athymic male mice. Approximately 3 weeks after cell implantation, the mice were treated with DTX or vehicle. Before and after the treatment, the mice were imaged with a microPET-Focus-F120 scanner (Concorde Microsystems, Knoxville, TN, USA) using FLT and (18)F-fluorodeoxyglucose (FDG). Tracer accumulations in the tumours were then analysed and compared with the proliferation activity and apoptotic index of the tumours. In a separate cell study, 22Rv1 cells were treated with DTX, then incubated with FLT or FDG and examined for their tracer uptake. RESULTS: The microPET imaging showed a significant decrease of FLT uptake in tumours after administration of DTX, while the changes of FDG uptake were minimal. Immunohistochemical analysis of the tumours revealed that the changes of FLT uptake were well correlated with those of proliferation activity but not with the apoptotic index. In vitro studies demonstrated that the significant decrease of FLT uptake in the cells after incubation with DTX correlated with the % S-phase cell fraction, while there were only minimal changes in the prostate-specific antigen concentration of the cell medium and FDG uptake in the cells. CONCLUSION: These results indicate that FLT is a promising tracer for monitoring the early effects of anticancer therapy with DTX in patients with HRPC. |
| | |
Authors:
|
Nobuyuki Oyama; Yoko Hasegawa; Yasushi Kiyono; Masato Kobayashi; Yasuhisa Fujibayashi; Datta E Ponde; Carmen Dence; Michael J Welch; Osamu Yokoyama |
Related Documents
:
|
1503903 - Synergism between a novel amphibian oocyte ribonuclease and lovastatin in inducing cyto... 6626263 - Methionine synthesis from 5'-methylthioadenosine by tumour cells. 18245793 - Electrical properties and gustatory responses of various taste disk cells of frog fungi... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-09-29 |
Journal Detail:
|
Title: European journal of nuclear medicine and molecular imaging Volume: 38 ISSN: 1619-7089 ISO Abbreviation: Eur. J. Nucl. Med. Mol. Imaging Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-21 Completed Date: 2011-04-06 Revised Date: 2013-05-28 |
Medline Journal Info:
|
Nlm Unique ID: 101140988 Medline TA: Eur J Nucl Med Mol Imaging Country: Germany |
Other Details:
|
Languages: eng Pagination: 81-9 Citation Subset: IM |
Affiliation:
|
Department of Urology, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuoka-Shimoaizuki, Fukui, 9101193, Japan. urono@u-fukui.ac.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antineoplastic Agents / pharmacology, therapeutic use* Biological Transport Cell Line, Tumor Cell Proliferation / drug effects Dideoxynucleosides / diagnostic use*, metabolism Disease Models, Animal Drug Evaluation, Preclinical Flow Cytometry Humans Immunohistochemistry Male Mice Positron-Emission Tomography Prostatic Neoplasms / drug therapy*, metabolism, pathology, radionuclide imaging* Taxoids / pharmacology, therapeutic use* Time Factors Treatment Outcome |
| Grant Support | |
ID/Acronym/Agency:
|
P30 CA91842/CA/NCI NIH HHS; U24 CA083060/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antineoplastic Agents; 0/Dideoxynucleosides; 0/Taxoids; 15H5577CQD/docetaxel; PG53R0DWDQ/alovudine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: PET, CT and MRI characterisation of the atherosclerotic plaque.
Next Document: Morphological variation in dentate and edentulous human mandibles.