Document Detail


Early response assessment in prostate carcinoma by ¹⁸F-fluorothymidine following anticancer therapy with docetaxel using preclinical tumour models.
MedLine Citation:
PMID:  20878403     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: The aim of the study was to assess the potential usefulness of 3-deoxy-3-(18)F-fluorothymidine (FLT) as a radiopharmaceutical for imaging the early therapeutic effects of docetaxel (DTX) on tumour proliferation in hormone-refractory prostate cancer (HRPC).
METHODS: Cells of the androgen-independent human prostate tumour cell line, 22Rv1, were implanted in athymic male mice. Approximately 3 weeks after cell implantation, the mice were treated with DTX or vehicle. Before and after the treatment, the mice were imaged with a microPET-Focus-F120 scanner (Concorde Microsystems, Knoxville, TN, USA) using FLT and (18)F-fluorodeoxyglucose (FDG). Tracer accumulations in the tumours were then analysed and compared with the proliferation activity and apoptotic index of the tumours. In a separate cell study, 22Rv1 cells were treated with DTX, then incubated with FLT or FDG and examined for their tracer uptake.
RESULTS: The microPET imaging showed a significant decrease of FLT uptake in tumours after administration of DTX, while the changes of FDG uptake were minimal. Immunohistochemical analysis of the tumours revealed that the changes of FLT uptake were well correlated with those of proliferation activity but not with the apoptotic index. In vitro studies demonstrated that the significant decrease of FLT uptake in the cells after incubation with DTX correlated with the % S-phase cell fraction, while there were only minimal changes in the prostate-specific antigen concentration of the cell medium and FDG uptake in the cells.
CONCLUSION: These results indicate that FLT is a promising tracer for monitoring the early effects of anticancer therapy with DTX in patients with HRPC.
Authors:
Nobuyuki Oyama; Yoko Hasegawa; Yasushi Kiyono; Masato Kobayashi; Yasuhisa Fujibayashi; Datta E Ponde; Carmen Dence; Michael J Welch; Osamu Yokoyama
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-09-29
Journal Detail:
Title:  European journal of nuclear medicine and molecular imaging     Volume:  38     ISSN:  1619-7089     ISO Abbreviation:  Eur. J. Nucl. Med. Mol. Imaging     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-21     Completed Date:  2011-04-06     Revised Date:  2013-10-01    
Medline Journal Info:
Nlm Unique ID:  101140988     Medline TA:  Eur J Nucl Med Mol Imaging     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  81-9     Citation Subset:  IM    
Affiliation:
Department of Urology, Faculty of Medical Sciences, University of Fukui, 23-3 Matsuoka-Shimoaizuki, Fukui, 9101193, Japan. urono@u-fukui.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / pharmacology,  therapeutic use*
Biological Transport
Cell Line, Tumor
Cell Proliferation / drug effects
Dideoxynucleosides / diagnostic use*,  metabolism
Disease Models, Animal
Drug Evaluation, Preclinical
Flow Cytometry
Humans
Immunohistochemistry
Male
Mice
Positron-Emission Tomography
Prostatic Neoplasms / drug therapy*,  metabolism,  pathology,  radionuclide imaging*
Taxoids / pharmacology,  therapeutic use*
Time Factors
Treatment Outcome
Grant Support
ID/Acronym/Agency:
P30 CA091842/CA/NCI NIH HHS; P30 CA91842/CA/NCI NIH HHS; U24 CA083060/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Dideoxynucleosides; 0/Taxoids; 15H5577CQD/docetaxel; PG53R0DWDQ/alovudine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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