Document Detail

Early quantitative method for measuring germination in non-green spores of Dryopteris paleacea using an epifluorescence-microscope technique.
MedLine Citation:
PMID:  11539034     Owner:  NASA     Status:  MEDLINE    
A method is described to determine germination by blue-light excited red fluorescence in the positively photoblastic spores of Dryopteris paleacea Sw. This fluorescence is due to chlorophyll as evidenced from 1) a fluorescence-emission spectrum in vivo, where a bright fluorescence around 675 nm is obtained only in red light (R)-irradiated spores and 2) in vitro measurements with acetone extracts prepared from homogenized spores. Significant amounts of chlorophyll can be found only in R-treated spores; this chlorophyll exhibits an emission band around 668 nm, when irradiated with 430 nm light at 21 degrees C. Compared to other criteria for germination, such as swelling of the cell, coat splitting, greening, and rhizoid formation, which require longer periods after induction for their expression, chlorophyll fluorescence can be used to quantify germination after two days. This result is confirmed by fluence-response curves for R-induced spore germination; the same relationship between applied R and germination is obtained by the evaluation with the epifluorescence method 2 days after the light treatment as compared with the evaluation with bright-field microscopy 5 days after the inducing R. Using this technique we show for the first time that Ca2+ contributes to the signal-transduction chain in phytochrome-mediated chlorophyll synthesis in spores of Dryopteris paleacea.
R Scheuerlein; R Wayne; S J Roux
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Physiologia plantarum     Volume:  73     ISSN:  0031-9317     ISO Abbreviation:  Physiol Plant     Publication Date:  1988  
Date Detail:
Created Date:  1996-12-16     Completed Date:  1996-12-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1256322     Medline TA:  Physiol Plant     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  505-11     Citation Subset:  S    
Institut für Botanik und Pharmazeutische Biologie, Universität Erlangen-Nürnberg, Erlangen, FRG.
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MeSH Terms
Calcium / physiology
Chlorophyll / metabolism*
Germination / physiology*,  radiation effects
Microscopy, Fluorescence / methods*
Plant Physiological Phenomena*
Plants / radiation effects
Spores / growth & development*,  radiation effects
Grant Support
Sche 276/1-1/SC/NCI NIH HHS
Reg. No./Substance:
1406-65-1/Chlorophyll; 7440-70-2/Calcium
S J Roux / U TX, Austin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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