| Early-phase endotoxic shock-induced myocardial injury increases iNOS and selectin expression in macaque primate. | |
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MedLine Citation:
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PMID: 17314070 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Our previous study has established a macaque model with early-phase endotoxic shock. The present study further investigated myocardial and blood vessel injury in Macaques by examining the subsequent expression of ACP, selectins, iNOS, and cTnI in response to LPS treatment. METHODS: In an experiment with anaesthetised, instrumental macaques, eleven animals were randomised into: an En group (n=6), receiving a dose of 2.8 mg kg(-1) lipopolysaccharides (LPS) by i.v.; and a Co group (n=5), injected with normal saline of 1 ml kg(-1). Cytochemistry of acid phosphatase (ACPase) in heart was performed by electron microscope at 120 min following endotoxin injection. Three immunochemical stains, namely, L-selectin, P-selectin and iNOS protein in heart, were studied. In addition, cardiac troponin I (cTnI), L-selectin and P-selectin in plasma were detected. RESULTS: In the early phase of endotoxic shock, LPS caused myocardial lysosome damage. The data of immunochemical staining showed the thrombus formation in vessels and the increase of iNOS, L-Selectin and P-Selectin expression in heart, but LPS challenge did not change L-selectin, P-selectin and cTnI in plasma. CONCLUSION: The increase of iNOS, L-selectin and P-selectin protein expression following endotoxin administration may have caused vessel injury and myocardial damage in macaques. |
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Authors:
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Guo Qing Yin; Kai He Du; Fei Rong Gu; Zhi Xun Fang; Jia Qi Tang; Bei Zhong; Xiao Yun Zhu; Ying Wei Wu; Cheng Ping Lu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-02-20 |
Journal Detail:
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Title: Heart, lung & circulation Volume: 16 ISSN: 1443-9506 ISO Abbreviation: Heart Lung Circ Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-03-23 Completed Date: 2007-06-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100963739 Medline TA: Heart Lung Circ Country: Australia |
Other Details:
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Languages: eng Pagination: 85-92 Citation Subset: IM |
Affiliation:
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College of Veterinary Medicine, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, PR China. yinguoq@jlonline.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal Escherichia coli Female L-Selectin / metabolism* Lipopolysaccharides Macaca Male Myocardium / metabolism*, pathology* Nitric Oxide Synthase Type II / metabolism* P-Selectin / metabolism* Shock, Septic / complications*, metabolism, pathology Troponin I / blood |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/P-Selectin; 0/Troponin I; 126880-86-2/L-Selectin; EC 1.14.13.39/Nitric Oxide Synthase Type II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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