Document Detail


Early morphological changes leading to central polydactyly, syndactyly, and central deficiencies: an experimental study in rats.
MedLine Citation:
PMID:  17996777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Various combinations of central polydactyly, syndactyly, and cleft hand have been frequently observed in the individual hands and feet in the same patients. Little is known, however, about the early changes of abnormal induction of digital rays during limb development. To determine the early changes and process of formation of central polydactyly, syndactyly, and cleft hand, we experimentally induced these anomalies in the hind limbs of rat embryos and discussed the relationship among these abnormalities. METHODS: Inbred WKAH/Hkm rats were used for this study. Pregnant females were treated with busulfan at embryonic day (E) 11. The embryos were removed at E12 to E21 and stained with alcian blue and alizarin red S. The abnormal changes in the treated embryos' hind limbs were observed with a microscope. RESULTS: The edges of the footplates were irregular, and their growth was reduced at E14. By E15, abnormal clefts in the distal edge were present that disrupted the central digits (2 to 4) of the footplates. Because of these abnormal clefts, the digital rays were bent or branched, and the neighboring interdigital spaces were narrowed. These changes led to central polydactyly, syndactyly, and central deficiencies. CONCLUSIONS: These findings show that central polydactyly, syndactyly, and central deficiencies have the same early morphological changes: abnormal clefts in the central part of the footplate.
Authors:
Takuji Naruse; Masatoshi Takahara; Michiaki Takagi; Toshihiko Ogino
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of hand surgery     Volume:  32     ISSN:  0363-5023     ISO Abbreviation:  J Hand Surg Am     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-11-12     Completed Date:  2008-02-06     Revised Date:  2009-06-08    
Medline Journal Info:
Nlm Unique ID:  7609631     Medline TA:  J Hand Surg Am     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1413-7     Citation Subset:  IM    
Affiliation:
Department of Orthopaedic Surgery, Yamagata University School of Medicine, Yamagata, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Alkylating / pharmacology
Busulfan / pharmacology
Embryo, Mammalian
Female
Hindlimb / embryology*
Humans
Inbreeding
Limb Deformities, Congenital / embryology*
Microscopy
Models, Animal
Pregnancy
Rats
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Alkylating; 55-98-1/Busulfan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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