Document Detail


Early morphological changes in the endothelium of a peripheral artery of rabbits fed an atherogenic diet.
MedLine Citation:
PMID:  6687574     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of an atherogenic diet on the endothelium of the central artery of the rabbit ear was studied by scanning and transmission electron microscopy. Examination of the inner surface of the artery after only 5 weeks on the diet revealed morphological changes including irregularly shaped cells, breaks at intercellular junctions, swelling of the membrane over the central part of the cells, and occasional holes in the cells. By 9 weeks more severe damage was seen including lifting off of cells and some holes. In addition, arrays of dark spots were seen by scanning electron microscopy in some cells in arteries of rabbits fed the diet for 5 to 9 weeks. The dark spots may correspond to abnormally large vacuoles seen inside endothelial cells by transmission electron microscopy. The central ear artery and aorta of some rabbits were tested for their ability to convert arachidonic acid into prostacyclin and thromboxane A2 by radioimmunoassay of the stable metabolites 6-keto-prostaglandin F1 alpha and thromboxane B2. The vessels from rabbits fed the atherogenic diet did not differ from vessels of rabbits on the control diet in their production of either metabolite.
Authors:
C M Ingerman-Wojenski; A W Sedar; M Nissenbaum; M J Silver; D M Klurfeld; D Kritchevsky
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental and molecular pathology     Volume:  38     ISSN:  0014-4800     ISO Abbreviation:  Exp. Mol. Pathol.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-05-05     Completed Date:  1983-05-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370711     Medline TA:  Exp Mol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  48-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
6-Ketoprostaglandin F1 alpha / biosynthesis
Animals
Aorta, Thoracic / metabolism
Arteries / metabolism,  pathology*
Arteriosclerosis / pathology*
Cell Membrane / pathology
Diet, Atherogenic*
Ear / blood supply
Endothelium / pathology*
Male
Microscopy, Electron
Rabbits
Thromboxane B2 / biosynthesis
Vacuoles / pathology
Grant Support
ID/Acronym/Agency:
HL-00734/HL/NHLBI NIH HHS; HL-03299/HL/NHLBI NIH HHS; HL-14890/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
54397-85-2/Thromboxane B2; 58962-34-8/6-Ketoprostaglandin F1 alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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