Document Detail


Early, microbially driven follicular reactions in the neonatal piglet do not contribute to expansion of the immunoglobulin heavy chain V-D-J repertoire.
MedLine Citation:
PMID:  17560662     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Selective microbial colonisation of germ-free piglets is reported to result in expansion of immunoglobulin V(H)- and D(H)-segment usage from an initially limited repertoire. Here, the response of the palatine tonsil to microbial colonisation was compared in age-matched conventionally reared and germ-free piglets. At 3 and 5 days after birth an expansion in the B-cell follicle area was observed in the conventional, microbially colonised animals, which was not seen in the germ-free piglets. Consistent with this observation, sequencing of re-arranged heavy chain V-D-J units demonstrated accumulation of point mutations indicating somatic hypermutation in the conventional, microbially colonised piglets but not in the germ-free animals. However, V(H)- and D(H)-segment usage and CDR3 length did not differ between the groups. The results suggest that the follicle reaction observed occurs in response to microbial challenge, involves proliferation and somatic hypermutation of B-cells but does not expand repertoire or generate classical, isotype-switched memory B-cells. We suggest that microbial colonisation of neonatal piglets drives immunological competence in two stages: first, an antigen non-specific, follicular reaction which expands immunological compartments; and second, microbe driven changes in V-segment usage which expand immunological repertoire.
Authors:
Stephen Wilson; Pam Norton; James Leigh; Michael Bailey
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-05
Journal Detail:
Title:  Veterinary immunology and immunopathology     Volume:  118     ISSN:  0165-2427     ISO Abbreviation:  Vet. Immunol. Immunopathol.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-26     Completed Date:  2007-09-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8002006     Medline TA:  Vet Immunol Immunopathol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  105-12     Citation Subset:  IM    
Affiliation:
Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford House, Bristol, United Kingdom. s_m_wilson@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antibody Diversity / genetics*,  physiology
B-Lymphocytes / immunology,  metabolism
Gene Rearrangement, B-Lymphocyte / genetics*
Germ-Free Life
Immunoglobulin Class Switching
Immunoglobulin Heavy Chains / biosynthesis,  genetics*,  immunology*
Palatine Tonsil / immunology
Swine / genetics,  immunology*,  microbiology*
Swine, Miniature
Chemical
Reg. No./Substance:
0/Immunoglobulin Heavy Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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