| Early maternal deprivation induces changes on the expression of 2-AG biosynthesis and degradation enzymes in neonatal rat hippocampus. | |
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MedLine Citation:
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PMID: 20599824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Early maternal deprivation (MD) in rats (24h, PND 9-10) is a model for neurodevelopmental stress. Our previous data showed that MD altered the hippocampal levels of the endocannabinoid 2-AG and the expression of hippocampal cannabinoid receptors in 13-day-old rats, with males being more markedly affected. The aim of this study was to analyze the impact of MD on the enzymes involved in 2-AG biosynthesis (DAGLalpha and DAGLbeta) and degradation (MAGL) in relevant areas (DG, CA1, CA3) of the hippocampus in 13-day-old neonatal rats. The expression of the enzymes was evaluated by quantitative RT-PCR, immunohistochemistry, and densitometry. MD induced a significant increase in DAGLalpha immunoreactivity in both males and females, which was mainly associated with fibers in the polymorphic cell layer of the dentate gyrus and in the stratum pyramidale of CA3. In contrast, the molecular layer of the dentate gyrus showed a significant decrease in DAGLalpha immunoreactivity in MD males and females. No changes were observed in DAGLbeta immunoreactivity. MD induced a significant decrease in MAGL immunoreactivity in hippocampal CA3 and CA1 areas, more marked in males than in females, and that was mainly associated with fibers in all strata of CA3 and CA1. The results also showed a significant decrease of MAGL mRNA levels in MD males. These data support a clear association between neurodevelopmental stress and dysregulation of the endocannabinoid system. This association may be relevant for schizophrenia and other neurodevelopmental psychiatric disorders. |
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Authors:
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Juan Suárez; Patricia Rivera; Ricardo Llorente; Silvana Y Romero-Zerbo; Francisco J Bermúdez-Silva; Fernando Rodríguez de Fonseca; María-Paz Viveros |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-25 |
Journal Detail:
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Title: Brain research Volume: 1349 ISSN: 1872-6240 ISO Abbreviation: Brain Res. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-03 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 162-73 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Laboratorio de Medicina Regenerativa, Fundación IMABIS, Hospital Carlos Haya, 29010 Málaga, Spain. juan.suarez@fundacionimabis.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Female Gene Expression Regulation, Developmental / physiology* Hippocampus / cytology, enzymology*, growth & development Lipoprotein Lipase / classification, genetics, metabolism* Male Maternal Deprivation* Monoacylglycerol Lipases / genetics, metabolism* Neurons / enzymology RNA, Messenger / metabolism Rats Rats, Wistar Sex Factors |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; EC 3.1.1.23/Monoacylglycerol Lipases; EC 3.1.1.34/Lipoprotein Lipase |
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