Document Detail


Early interactions of marine birnavirus infection in several fish cell lines.
MedLine Citation:
PMID:  12810875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Marine birnavirus (MABV), a member of the genus Aquabirnavirus, family Birnaviridae, is an unenveloped icosahedral virus with two genomes of double-stranded RNA. The mechanisms of MABV adsorption and penetration are still undetermined. This work examined MABV infection in susceptible and resistant fish cell lines. MABV adsorbed not only onto the cell surfaces of susceptible (CHSE-214 and RSBK-2) cells but also onto resistant (FHM and EPC) cells. Furthermore, the virus entered the cytoplasm through the endocytotic pathway in CHSE-214, RSBK-2 and FHM cells but did not penetrate EPC cells. Thus, restriction of the MABV replication cycle is different between resistant FHM and EPC cells. The virus was found to bind to an around 250 kDa protein on CHSE-214, RSBK-2, FHM and EPC cells. Thus, this 250 kDa protein may be a major MABV receptor that exists in the plasma membranes of all four cell lines examined. This result suggests further that another receptor for virus penetration may exist in CHSE-214, RSBK-2 and FHM cells but not in EPC cells.
Authors:
Masayuki Imajoh; Ken-ichi Yagyu; Syun-ichirou Oshima
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general virology     Volume:  84     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-17     Completed Date:  2003-08-27     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1809-16     Citation Subset:  IM    
Affiliation:
Department of Aquaculture Kochi University, Nankoku, Kochi 783-8502, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aquabirnavirus / isolation & purification,  metabolism,  pathogenicity*
Birnaviridae Infections / virology*
Cell Line / ultrastructure
Cyprinidae / virology
Disease Susceptibility
Endocytosis
Fishes / virology*
Receptors, Virus / metabolism
Salmon / virology
Sea Bream / virology
Seawater
Chemical
Reg. No./Substance:
0/Receptors, Virus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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