Document Detail

Early interactions of cancer cells with the microvasculature in mouse liver and muscle during hematogenous metastasis: videomicroscopic analysis.
MedLine Citation:
PMID:  8375113     Owner:  NLM     Status:  MEDLINE    
Biomechanical interactions of cancer cells with the microvasculature were studied using high resolution intravital videomicroscopy. We compared initial arrest of murine B16F10 melanoma and D2A1 mammary carcinoma cells fluorescently labelled with calcein-AM, in low pressure (liver) vs high pressure (cremaster muscle) microvascular beds. Cells were arrested due to size restriction at the inflow side of the microcirculation, penetrating further and becoming more deformed in muscle than liver [median length to width ratios of 3.3 vs 1.3 for D2A1 cells, and 2.5 vs 1.2 for B16F10, at 1 min post-injection (p.i.)]. During the next 2 h many cells became stretched, giving maximum length to width ratios of 68 vs 22.1 (D2A1) and 28 vs 5.6 (B16F10) in muscle vs liver. Ethidium bromide exclusion demonstrated that over 97% of the cells maintained membrane integrity for > 2 h p.i. (In contrast, when an acridine orange labelling procedure was used, membrane disruption of B16F10 cells occurred within 15 min p.i.) Our experiments do not indicate the ultimate fate of the cancer cells, but if cell lysis occurs it must be on a time scale of hours rather than minutes. We report a process of 'clasmatosis' in cancer cells arrested in the microcirculation: large membrane-enclosed fragments (> 3 microns in diameter) became 'pinched off' from arrested cells, in both liver and muscle, often within minutes or even seconds of arrest. The significance of this process is not yet understood. In this study intravital videomicroscopy has thus provided a valuable clarification of the interactions of cancer cells with vessel walls during metastasis.
V L Morris; I C MacDonald; S Koop; E E Schmidt; A F Chambers; A C Groom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical & experimental metastasis     Volume:  11     ISSN:  0262-0898     ISO Abbreviation:  Clin. Exp. Metastasis     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-10-18     Completed Date:  1993-10-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8409970     Medline TA:  Clin Exp Metastasis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  377-90     Citation Subset:  IM    
Department of Microbiology and Immunology, University of Western Ontario, London, Canada.
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MeSH Terms
Chick Embryo
Liver / pathology
Mammary Neoplasms, Experimental / pathology*
Melanoma, Experimental / pathology*
Muscles / pathology
Neoplasm Metastasis*
Neoplasm Transplantation
Video Recording
Comment In:
Clin Exp Metastasis. 1993 Sep;11(5):430-6   [PMID:  7690692 ]

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