Early initiation of DNA synthesis in G1 phase HeLa cells following fusion with red cell ghosts loaded with S-phase cell extracts. | |
MedLine Citation:
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PMID: 3880703 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A modification of polyethylene glycol-mediated cell fusion procedure has been described and standardized for red blood cell mediated microinjection of proteins into cells in suspension. Using this procedure, proteins are routinely introduced into 50% of the target cells. We have applied this microinjection procedure to introduce cytoplasmic extracts obtained from S-phase HeLa cells into HeLa cells synchronized in G1. This experiment resulted in an accelerated entry of the G1 cells into S phase as measured by the incorporation of [3H]thymidine. This technique may provide a means to identify and characterize the S-phase factors responsible for the induction of DNA synthesis. |
Authors:
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D B Brown; S K Hanks; E C Murphy; P N Rao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Experimental cell research Volume: 156 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1985 Jan |
Date Detail:
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Created Date: 1985-02-04 Completed Date: 1985-02-04 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 251-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle Cell Fusion DNA Replication* Erythrocyte Membrane / ultrastructure* Fluorescent Antibody Technique Hela Cells / cytology, metabolism Humans Interphase* Kinetics Mitosis Ovalbumin / analysis Tissue Extracts / pharmacology |
Grant Support | |
ID/Acronym/Agency:
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CA 27544/CA/NCI NIH HHS; CA 34783/CA/NCI NIH HHS |
Chemical | |
Reg. No./Substance:
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0/Tissue Extracts; 9006-59-1/Ovalbumin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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