Document Detail


Early initiation of DNA synthesis in G1 phase HeLa cells following fusion with red cell ghosts loaded with S-phase cell extracts.
MedLine Citation:
PMID:  3880703     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modification of polyethylene glycol-mediated cell fusion procedure has been described and standardized for red blood cell mediated microinjection of proteins into cells in suspension. Using this procedure, proteins are routinely introduced into 50% of the target cells. We have applied this microinjection procedure to introduce cytoplasmic extracts obtained from S-phase HeLa cells into HeLa cells synchronized in G1. This experiment resulted in an accelerated entry of the G1 cells into S phase as measured by the incorporation of [3H]thymidine. This technique may provide a means to identify and characterize the S-phase factors responsible for the induction of DNA synthesis.
Authors:
D B Brown; S K Hanks; E C Murphy; P N Rao
Related Documents :
1168203 - The effect of potassium on the cell membrane potential and the passage of synchronized ...
1561313 - Cell-cycle-dependent radiation-induced oncogenic transformation of c3h 10t1/2 cells.
6734733 - Centrosomes and mitotic poles.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  156     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1985 Jan 
Date Detail:
Created Date:  1985-02-04     Completed Date:  1985-02-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  251-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Cycle
Cell Fusion
DNA Replication*
Erythrocyte Membrane / ultrastructure*
Fluorescent Antibody Technique
Hela Cells / cytology,  metabolism
Humans
Interphase*
Kinetics
Mitosis
Ovalbumin / analysis
Tissue Extracts / pharmacology
Grant Support
ID/Acronym/Agency:
CA 27544/CA/NCI NIH HHS; CA 34783/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Tissue Extracts; 9006-59-1/Ovalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Interaction of triethyl lead chloride with microtubules in vitro and in mammalian cells.
Next Document:  Rapid determination of DNA synthesis in adherent cells grown in microtiter plates.