| Early gene regulation of osteogenesis in embryonic stem cells. | |
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MedLine Citation:
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PMID: 23042286 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The early gene regulatory networks (GRNs) that mediate stem cell differentiation are complex, and the underlying regulatory associations can be difficult to map accurately. In this study, the expression profiles of the genes Dlx5, Msx2 and Runx2 in mouse embryonic stem cells were monitored over a 48 hour period after exposure to the growth factors BMP2 and TGFβ1. Candidate GRNs of early osteogenesis were constructed based on published experimental findings and simulation results of Boolean and ordinary differential equation models were compared with our experimental data in order to test the validity of these models. Three gene regulatory networks were found to be consistent with the data, one of these networks exhibited sustained oscillation, a behaviour which is consistent with the general view of embryonic stem cell plasticity. The work cycle presented in this paper illustrates how mathematical modelling can be used to elucidate from gene expression profiles GRNs that are consistent with experimental data. |
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Authors:
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Glen R Kirkham; Anna Lovrics; Helen M Byrne; Oliver E Jensen; John R King; Kevin M Shakesheff; Lee D K Buttery |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Integrative biology : quantitative biosciences from nano to macro Volume: 4 ISSN: 1757-9708 ISO Abbreviation: Integr Biol (Camb) Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-11-20 Completed Date: 2013-04-26 Revised Date: 2013-06-12 |
Medline Journal Info:
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Nlm Unique ID: 101478378 Medline TA: Integr Biol (Camb) Country: England |
Other Details:
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Languages: eng Pagination: 1470-7 Citation Subset: IM |
Affiliation:
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Centre for Biomolecular Sciences, University Park, Nottingham, UK. glen.kirkham@nottingham.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Bone Morphogenetic Protein 2 / pharmacology Cell Differentiation / drug effects, genetics Cell Line Core Binding Factor Alpha 1 Subunit / genetics Embryonic Stem Cells / cytology*, drug effects, metabolism* Gene Expression Regulation, Developmental Gene Regulatory Networks Homeodomain Proteins / genetics Mice Models, Biological Osteogenesis / drug effects, genetics*, physiology RNA / genetics, metabolism Systems Biology Transcriptome Transforming Growth Factor beta1 / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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BB/D008522/1//Biotechnology and Biological Sciences Research Council |
| Chemical | |
Reg. No./Substance:
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0/Bmp2 protein, mouse; 0/Bone Morphogenetic Protein 2; 0/Core Binding Factor Alpha 1 Subunit; 0/Dlx5 protein, mouse; 0/Homeodomain Proteins; 0/MSX2 protein; 0/Runx2 protein, mouse; 0/Transforming Growth Factor beta1; 63231-63-0/RNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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