Document Detail

Early gene regulation of osteogenesis in embryonic stem cells.
MedLine Citation:
PMID:  23042286     Owner:  NLM     Status:  MEDLINE    
The early gene regulatory networks (GRNs) that mediate stem cell differentiation are complex, and the underlying regulatory associations can be difficult to map accurately. In this study, the expression profiles of the genes Dlx5, Msx2 and Runx2 in mouse embryonic stem cells were monitored over a 48 hour period after exposure to the growth factors BMP2 and TGFβ1. Candidate GRNs of early osteogenesis were constructed based on published experimental findings and simulation results of Boolean and ordinary differential equation models were compared with our experimental data in order to test the validity of these models. Three gene regulatory networks were found to be consistent with the data, one of these networks exhibited sustained oscillation, a behaviour which is consistent with the general view of embryonic stem cell plasticity. The work cycle presented in this paper illustrates how mathematical modelling can be used to elucidate from gene expression profiles GRNs that are consistent with experimental data.
Glen R Kirkham; Anna Lovrics; Helen M Byrne; Oliver E Jensen; John R King; Kevin M Shakesheff; Lee D K Buttery
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  4     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-20     Completed Date:  2013-04-26     Revised Date:  2013-06-12    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1470-7     Citation Subset:  IM    
Centre for Biomolecular Sciences, University Park, Nottingham, UK.
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MeSH Terms
Base Sequence
Bone Morphogenetic Protein 2 / pharmacology
Cell Differentiation / drug effects,  genetics
Cell Line
Core Binding Factor Alpha 1 Subunit / genetics
Embryonic Stem Cells / cytology*,  drug effects,  metabolism*
Gene Expression Regulation, Developmental
Gene Regulatory Networks
Homeodomain Proteins / genetics
Models, Biological
Osteogenesis / drug effects,  genetics*,  physiology
RNA / genetics,  metabolism
Systems Biology
Transforming Growth Factor beta1 / pharmacology
Grant Support
BB/D008522/1//Biotechnology and Biological Sciences Research Council
Reg. No./Substance:
0/Bmp2 protein, mouse; 0/Bone Morphogenetic Protein 2; 0/Core Binding Factor Alpha 1 Subunit; 0/Dlx5 protein, mouse; 0/Homeodomain Proteins; 0/MSX2 protein; 0/Runx2 protein, mouse; 0/Transforming Growth Factor beta1; 63231-63-0/RNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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