| Early expression of injury-induced neuropeptide Y in primary sensory neurons and the cuneate nucleus in diabetic rats with median nerve transection. | |
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MedLine Citation:
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PMID: 20658772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study we examined the temporal changes in neuropeptide Y (NPY) expression in dorsal root ganglion (DRG) neurons and cuneate nucleus (CN) in streptozotocin (STZ)-induced diabetic rats with or without median nerve transection (MNT). Numerous NPY-like immunoreactive (NPY-LI) neurons and fibers were detected in the DRG and CN of the diabetic MNT (DMNT) rats respectively, but not in those with diabetes-alone. Following MNT, the time-course of NPY expression pattern in the diabetic DRG and CN was similar and both peaked at 2 weeks, which was earlier than those in the non-diabetic MNT rats. Consequently, the expression of neurotrophin-3 (NT-3) immunoreactivity in DRG neurons was coincidentally decreased and reached the nadir at 2 weeks in the diabetic MNT rats, which was also earlier than that in the non-diabetic MNT rats. Following electrical stimulation of the injured nerves, the number of NPY-LI fibers became attenuated and the induced c-Fos-LI cells concurrently appeared in the ipsilateral CN. In the diabetic CN, the number of c-Fos-LI cells also peaked at 2 weeks after MNT, which was consistent with the temporal pattern of changes in NPY expression. The results suggest that in diabetes, MNT induced NPY expression via the reduction of NT-3, and electrical stimulation of the injured median nerve evoked the release of NPY and accordingly more c-Fos-LI cells were identified in the CN. Furthermore, this study demonstrated early NPY and c-Fos expression in the diabetic rats after MNT, suggesting that the development of neuropathic signs may be advanced in hyperglycemic rats. |
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Authors:
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Chi-Te Lin; Yi-Ju Tsai; Seu-Hwa Chen; Hsin-Ying Wang; Lung-Huang Lin; June-Horng Lue |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chemical neuroanatomy Volume: 40 ISSN: 1873-6300 ISO Abbreviation: J. Chem. Neuroanat. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-07-23 Completed Date: 2010-10-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8902615 Medline TA: J Chem Neuroanat Country: Netherlands |
Other Details:
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Languages: eng Pagination: 102-11 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Diabetes Mellitus, Experimental / metabolism* Electric Stimulation Ganglia, Spinal / metabolism* Immunohistochemistry Male Median Nerve / injuries*, metabolism Medulla Oblongata / metabolism* Microscopy, Confocal Neuropeptide Y / metabolism* Neurotrophin 3 / metabolism Proto-Oncogene Proteins c-fos / metabolism Rats Rats, Sprague-Dawley Sensory Receptor Cells / metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Neuropeptide Y; 0/Neurotrophin 3; 0/Proto-Oncogene Proteins c-fos |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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