Document Detail


Early events of the exogenously provided L-Carnitine in murine macrophages, T- and B-lymphocytes: modulation of prostaglandin E1 and E2 production in response to arachidonic acid.
MedLine Citation:
PMID:  12873717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
L-carnitine is an essential energy-providing compound to the cell since it transports long chain fatty acids through the mitochondrial membrane and delivers them to the beta-oxidation pathway for catabolism and/or entrance to biosynthetic pathways. Some of the early events taking place in immune cells after L-carnitine inoculation in vitro are defined in this report. Using arachidonic acid as a fatty acid source, we determined the utilization rate of L-carnitine by murine T-, B-lymphocytes and macrophages within two hours of cell culture, its effect on prostaglandin E1 and E2 production and the levels of beta-hydroxy-butyrate. The results show that although all immune cells consume a small portion of L-carnitine, beta-hydroxy-butyrate decreases upon addition of arachidonic acid and/or L-carnitine indicating that active biosynthetic pathways are induced. L-carnitine is shown to increase the arachidonic acid-induced production of prostaglandins E1 and E2 in macrophages, while their secretion from T- and B-lymphocytes is decreased. These findings indicate the L-carnitine may very rapidly alter the activation state of immune cells and lead to the development of various reactions, beneficial or not to the organism.
Authors:
Irene Athanassakis; Eva Dionyssopoulou; Sunny Papanikou; Athanassios Evangeliou; Simon Vassiliadis
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  14     ISSN:  0955-2863     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-09-03     Completed Date:  2003-11-07     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  350-7     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Crete, Heraklion, Crete, Greece. athan@biology.uoc.gr
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MeSH Terms
Descriptor/Qualifier:
3-Hydroxybutyric Acid / analysis,  metabolism
Alprostadil / biosynthesis*
Animals
Arachidonic Acid / pharmacology*
B-Lymphocytes / drug effects,  metabolism
Carnitine / metabolism,  pharmacology*
Cells, Cultured
Dinoprostone / biosynthesis*
Drug Interactions
Kinetics
Lymphocytes / drug effects*,  metabolism
Macrophages / drug effects*,  metabolism
Mice
Mice, Inbred BALB C
T-Lymphocytes / drug effects,  metabolism
Chemical
Reg. No./Substance:
300-85-6/3-Hydroxybutyric Acid; 363-24-6/Dinoprostone; 506-32-1/Arachidonic Acid; 541-15-1/Carnitine; 745-65-3/Alprostadil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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