Document Detail

Early effects of extracorporeal shock wave treatment on osteoblast-like cells: a comparative study between electromagnetic and electrohydraulic devices.
MedLine Citation:
PMID:  17099529     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Extracorporeal shockwave therapy (ESWT) has been increasingly applied to treat orthopedic and musculoskeletal pathologies. ESWT involves mechanical perturbations that, as with other physical therapies, can result in mechanical stimuli to a large number of cells, including bone cells. The aim of this study was to evaluate the effects of shock waves on osteoblast-like cells (MG63) when using two different generators of shock waves (electrohydraulic and electromagnetic devices), in terms of cell damage, cell viability, osteogenic phenotype expression, and cytokine production. METHODS: MG63 cells were suspended in 1.5 mL screw-cap cryotubes (1 x 10 cells/mL), containing phosphate buffer solution (PBS), which were maintained at 37 degrees C during all the experimental times. Two levels of energy flux density (EFD) were evaluated for each device: 0.15 to 0.18 mJ/mm2 and 0.40 mJ/mm2. Cells were then cultivated for 72 hours starting from a concentration of 1 x 10 cells/mL, and biological activity and viability were evaluated 24 and 72 hours after treatment. RESULTS: The results obtained demonstrate that the factors most affecting osteoblast activity involve both the device and the level of EFD selected, and they must be considered all together. CONCLUSIONS: The use of the electromagnetic device and a level of EFD lower than 0.40 mJ/mm2 would appear to induce fewer immediate cytodestructive effects and better stimulate subsequent proliferation and the synthetic activity of MG63.
Lucia Martini; Gianluca Giavaresi; Milena Fini; Veronica Borsari; Paola Torricelli; Roberto Giardino
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of trauma     Volume:  61     ISSN:  0022-5282     ISO Abbreviation:  J Trauma     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-13     Completed Date:  2006-12-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376373     Medline TA:  J Trauma     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1198-206     Citation Subset:  AIM; IM    
Experimental Surgery Department, Rizzoli Orthopaedic Institute, Bologna, Italy.
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MeSH Terms
Cell Survival / physiology
Cells, Cultured
Dose-Response Relationship, Radiation
Electromagnetic Phenomena*
High-Energy Shock Waves*
Osteoblasts / radiation effects*
Time Factors

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