| Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots. | |
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MedLine Citation:
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PMID: 20211580 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: More routine laboratories in South Africa are equipped to perform quantitative than qualitative HIV viral detection assays. The accessibility of early infant diagnosis would be improved if a quantitative viral load (VL) assay performed on dried blood spots (DBS) could accurately diagnose HIV-infection. The VL assay routinely used in the country has not previously been assessed on DBS for early infant diagnosis. OBJECTIVES: To determine the accuracy of the NucliSens EasyQ assay (bioMerieux, Lyon, France) on DBS for early infant diagnosis of HIV in a subtype C-infected population. STUDY DESIGN: Stored DBS samples collected from children presenting for HIV testing were analyzed. DBS EasyQ VL results were compared to the child's HIV status as determined by a whole blood HIV DNA PCR result. RESULTS: The EasyQ assay was performed on 235 stored DBS samples from 71 HIV-infected and 164 HIV-uninfected children. Of the 216 infants (children aged 12 months or less) tested, all 52 HIV-infected infants were detected (sensitivity of 100%). Six of 164 HIV-uninfected infants yielded false positive results (specificity 96.3%). All false positive and six of the true positive infants had VL<3.7 log IU/ml. These 12 (5.6%) infants would require repeat testing to differentiate true from false positives. Using a threshold above 3.7 log IU/ml (equivalent to 4 log copies/ml) to define a positive result would yield an accurate diagnosis in 204 (94.4%) infants. CONCLUSIONS: DBS EasyQ VL assays provide an accurate option for early infant diagnosis in low resource settings. |
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Authors:
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R R Lilian; K Bhowan; G G Sherman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-07 |
Journal Detail:
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Title: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Volume: 48 ISSN: 1873-5967 ISO Abbreviation: J. Clin. Virol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-16 Completed Date: 2010-07-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815671 Medline TA: J Clin Virol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 40-3 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Paediatric HIV Diagnostic Syndicate, Wits Health Consortium, 8 Blackwood Avenue, Parktown, Johannesburg, South Africa. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Data Interpretation, Statistical False Positive Reactions HIV Infections / diagnosis, virology* HIV-1 / genetics* Humans Infant Nucleic Acid Amplification Techniques / methods* RNA, Viral / blood Reagent Kits, Diagnostic* Sensitivity and Specificity Specimen Handling / methods Viral Load |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral; 0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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