Document Detail

Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots.
MedLine Citation:
PMID:  20211580     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: More routine laboratories in South Africa are equipped to perform quantitative than qualitative HIV viral detection assays. The accessibility of early infant diagnosis would be improved if a quantitative viral load (VL) assay performed on dried blood spots (DBS) could accurately diagnose HIV-infection. The VL assay routinely used in the country has not previously been assessed on DBS for early infant diagnosis. OBJECTIVES: To determine the accuracy of the NucliSens EasyQ assay (bioMerieux, Lyon, France) on DBS for early infant diagnosis of HIV in a subtype C-infected population. STUDY DESIGN: Stored DBS samples collected from children presenting for HIV testing were analyzed. DBS EasyQ VL results were compared to the child's HIV status as determined by a whole blood HIV DNA PCR result. RESULTS: The EasyQ assay was performed on 235 stored DBS samples from 71 HIV-infected and 164 HIV-uninfected children. Of the 216 infants (children aged 12 months or less) tested, all 52 HIV-infected infants were detected (sensitivity of 100%). Six of 164 HIV-uninfected infants yielded false positive results (specificity 96.3%). All false positive and six of the true positive infants had VL<3.7 log IU/ml. These 12 (5.6%) infants would require repeat testing to differentiate true from false positives. Using a threshold above 3.7 log IU/ml (equivalent to 4 log copies/ml) to define a positive result would yield an accurate diagnosis in 204 (94.4%) infants. CONCLUSIONS: DBS EasyQ VL assays provide an accurate option for early infant diagnosis in low resource settings.
R R Lilian; K Bhowan; G G Sherman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-07
Journal Detail:
Title:  Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology     Volume:  48     ISSN:  1873-5967     ISO Abbreviation:  J. Clin. Virol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-16     Completed Date:  2010-07-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9815671     Medline TA:  J Clin Virol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  40-3     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Paediatric HIV Diagnostic Syndicate, Wits Health Consortium, 8 Blackwood Avenue, Parktown, Johannesburg, South Africa.
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MeSH Terms
Data Interpretation, Statistical
False Positive Reactions
HIV Infections / diagnosis,  virology*
HIV-1 / genetics*
Nucleic Acid Amplification Techniques / methods*
RNA, Viral / blood
Reagent Kits, Diagnostic*
Sensitivity and Specificity
Specimen Handling / methods
Viral Load
Reg. No./Substance:
0/RNA, Viral; 0/Reagent Kits, Diagnostic

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