Document Detail


Early developmental expression of Mus musculus zinc finger RNA-binding protein compared to orthologs in Caenorhabditis elegans and Danio rerio and subcellular localization of Mus musculus and Caenorhabditis elegans zinc finger RNA-binding protein in 2-cell Mus musculus embryos.
MedLine Citation:
PMID:  21091219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In mouse, knock-out of the Zfr gene encoding the zinc finger RNA-binding protein (ZFR) is associated with early lethality during gastrulation, suggesting that a pool of maternally contributed Zfr mRNA might compensate to allow development. ZFR is an ancient and highly conserved chromosome-associated protein from nematodes to mammals. We characterized expression of the Zfr transcript during early development in Mus musculus, Danio rerio, and Caenorhabditis elegans by quantitative real-time polymerase chain reaction. Mouse Zfr mRNA was detected in all stages tested during mouse preimplantation, with higher levels at the 1-cell stage that includes the maternal contribution of Zfr mRNA. In D. rerio, Zfr mRNA expression was highest in unfertilized eggs and declines throughout development. In C. elegans, Zfr mRNA expression was barely detectable in the fertilized egg and the L1 stage, but increased in the adult organism. Microinjections of green fluorescent protein (GFP)-tagged versions of in vitro-transcribed mouse and C. elegans Zfr mRNAs into early mouse embryos allowed analysis of the intracellular localization of the protein. Mouse ZFR-GFP was localized in the nucleus in 2-cell stage embryos although absent from nucleoli. Deletion studies revealed that this nuclear localization required the C-terminal part of ZFR, as deletion of the C-terminal resulted in the localization to the nuclear membrane. Despite the lack of a conserved nuclear localization signal, the C. elegans ZFR-GFP fusion protein also displayed an intranuclear localization in the 2-cell mouse embryo.
Authors:
Canan Doğanlı; Tine Kjærgaard; Anders Olsen; Claus Oxvig; Ernst-Martin Füchtbauer; Karin Lykke-Hartmann
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Publication Detail:
Type:  Journal Article     Date:  2010-11-22
Journal Detail:
Title:  DNA and cell biology     Volume:  29     ISSN:  1557-7430     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-14     Completed Date:  2011-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  713-27     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, Aarhus University, Aarhus C, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / embryology*,  genetics
Caenorhabditis elegans Proteins / biosynthesis*,  genetics
Female
Gene Expression Regulation, Developmental*
Humans
Male
Mice
Mice, Inbred C57BL
RNA-Binding Proteins / biosynthesis*,  genetics
Sequence Deletion
Sequence Homology, Amino Acid
Transcription, Genetic
Zebrafish / embryology*,  genetics
Zebrafish Proteins / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/Caenorhabditis elegans Proteins; 0/RNA-Binding Proteins; 0/ZFR protein, mouse; 0/Zebrafish Proteins; 0/Zfr protein, C elegans; 0/Zfr protein, zebrafish

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