Document Detail


Early detection of cell-free fetal DNA in maternal plasma.
MedLine Citation:
PMID:  17234369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: We aimed to establish the earliest gestational age at which fetal DNA in maternal plasma could be detected and whether this was reliable at 12-13 weeks' gestation. STUDY DESIGN: A prospective observational cohort study of 32 pregnancies either after IVF or before prenatal diagnosis by CVS. Maternal blood was taken and RT-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed at delivery or on karyotype. RESULTS: Y signal was obtained as early as 14 days post conception (4 weeks' gestation) and has a good prediction rate by 12 weeks' gestation. CONCLUSION: Free fetal DNA allows very early prediction of fetal sex in some cases and could be useful for clinical use for X-linked conditions by the end of the first trimester.
Authors:
S Illanes; M Denbow; C Kailasam; K Finning; P W Soothill
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Publication Detail:
Type:  Journal Article     Date:  2007-01-16
Journal Detail:
Title:  Early human development     Volume:  83     ISSN:  0378-3782     ISO Abbreviation:  Early Hum. Dev.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-17     Completed Date:  2007-11-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708381     Medline TA:  Early Hum Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  563-6     Citation Subset:  IM    
Affiliation:
Fetal Medicine Research Unit, Division of Obstetrics and Gynaecology, University of Bristol, Bristol, UK. s.illanes@bristol.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Adult
Chorionic Villi Sampling
Chromosomes, Human, Y*
DNA / blood*
Female
Gestational Age*
Humans
Male
Predictive Value of Tests
Pregnancy
Pregnancy Trimester, First / blood
Prospective Studies
Reproducibility of Results
Sex Determination (Analysis) / methods*
Sperm Injections, Intracytoplasmic
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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