| Early detachment of colon carcinoma cells during CD95(APO-1/Fas)-mediated apoptosis. I. De-adhesion from hyaluronate by shedding of CD44. | |
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MedLine Citation:
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PMID: 8769429 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ligation of CD95 (APO-1/Fas) cell surface receptors induces death in apoptosis-sensitive cells. Induction of apoptosis in adherent gamma interferon-stimulated HT-29 and COLO 205 colon carcinoma cells by cross-linking CD95 with anti-APO-1 monoclonal antibody resulted in detachment of the cells from hyaluronate starting about 1 h after antibody exposure. Loss of adhesion was paralleled by a substantial reduction of the multifunctional cell surface adhesion molecule CD44. As evidenced by cycloheximide treatment, this effect was not caused by impaired protein synthesis. Depletion of surface CD44 was also not due to membrane blebbing, since cytochalasin B failed to inhibit ascension from hyaluronate. Instead, ELISA and time kinetics showed increasing amounts of soluble CD44 in the supernatant of CD95-triggered cells. SDS-PAGE revealed that soluble CD44 had an apparent molecular mass of about 20 kD less than CD44 immunoprecipitated from intact cells. Thus, CD95-triggering induced shedding of CD44. Shedding is a novel mechanism operative in early steps of CD95-mediated apoptosis. Shedding surface molecules like CD44 might contribute to the active disintegration of dying epithelial cells in vivo. |
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Authors:
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A R Günthert; J Sträter; U von Reyher; C Henne; S Joos; K Koretz; G Moldenhauer; P H Krammer; P Möller |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of cell biology Volume: 134 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-10-01 Completed Date: 1996-10-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1089-96 Citation Subset: IM |
Affiliation:
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Institute of Pathology, University of Heidelberg, Germany. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD44
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chemistry,
metabolism* Antigens, CD95 / physiology* Apoptosis / physiology* Carcinoma Cell Adhesion / physiology* Colonic Neoplasms Cycloheximide / pharmacology Cytochalasin B / pharmacology DNA, Neoplasm / analysis Epithelial Cells Epithelium / metabolism HT29 Cells Humans Hyaluronic Acid / pharmacology Kinetics Molecular Weight Protease Inhibitors / pharmacology Protein Synthesis Inhibitors / pharmacology Solubility Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD44; 0/Antigens, CD95; 0/DNA, Neoplasm; 0/Protease Inhibitors; 0/Protein Synthesis Inhibitors; 14930-96-2/Cytochalasin B; 66-81-9/Cycloheximide; 9004-61-9/Hyaluronic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 2 Month: 8 Year: 1996 Volume: 134 Issue: 4 First Page: 1089 Last Page: 1096 ID: 2120951 Publisher Id: 96354922 PubMed Id: 8769429 |
| Early detachment of colon carcinoma cells during CD95(APO-1/Fas)- mediated apoptosis. I. De-adhesion from hyaluronate by shedding of CD44 | |
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