Document Detail

Early demethylation of non-CpG, CpC-rich, elements in the myogenin 5'-flanking region: a priming effect on the spreading of active demethylation.
MedLine Citation:
PMID:  20935518     Owner:  NLM     Status:  MEDLINE    
The dynamic changes and structural patterns of DNA methylation of genes without CpG islands are poorly characterized. The relevance of CpG to the non-CpG methylation equilibrium in transcriptional repression is unknown. In this work, we analyzed the DNA methylation pattern of the 5'-flanking of the myogenin gene, a positive regulator of muscle differentiation with no CpG island and low CpG density, in both C2C12 muscle satellite cells and embryonic muscle. Embryonic brain was studied as a non-expressing tissue. High levels of both CpG and non-CpG methylation were observed in non-expressing experimental conditions. Both CpG and non-CpG methylation rapidly dropped during muscle differentiation and myogenin transcriptional activation, with an active demethylation dynamics. Non-CpG demethylation occurred more rapidly than CpG demethylation. Demethylation spread from initially highly methylated short CpC-rich elements to a virtually unmethylated status. These short elements have a high CpC content and density, share some motifs and largely coincide with putative recognition sequences of some differentiation-related transcription factors. Our findings point to a dynamically controlled equilibrium between CpG and non-CpG active demethylation in the transcriptional control of tissue-specific genes. The short CpC-rich elements are new structural features of the methylation machinery, whose functions may include priming the complete demethylation of a transcriptionally crucial DNA region.
Andrea Fuso; Giampiero Ferraguti; Francesco Grandoni; Raffaella Ruggeri; Sigfrido Scarpa; Roberto Strom; Marco Lucarelli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-10-29
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  9     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-27     Completed Date:  2011-03-02     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3965-76     Citation Subset:  IM    
Department of Surgery P. Valdoni, Sapienza University of Rome, Italy.
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MeSH Terms
5' Flanking Region / genetics*
Base Sequence
Cell Line
Cluster Analysis
CpG Islands*
DNA Methylation*
Gene Expression Regulation
Molecular Sequence Data
Muscle Development / physiology
Muscle, Skeletal / cytology,  physiology
Myogenin / genetics*,  metabolism
Promoter Regions, Genetic
Reg. No./Substance:
Comment In:
Cell Cycle. 2010 Oct 1;9(19):3846-7   [PMID:  20948285 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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