| Early changes of protein synthesis in myocardium and coronary arteries induced by NO synthase inhibition. | |
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MedLine Citation:
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PMID: 10453747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The question was addressed whether short-term (4 hour) NO deficiency, inducing an increase in blood pressure in anaesthetized dogs, does influence proteosynthesis in the myocardium and coronary arteries. A potentially positive answer was to be followed by the study of the supporting role of ornithine decarboxylase for the polyamines pathway. N(G)-nitro-L-arginine-methyl ester (L-NAME) (50 mg/kg per hour) was administered i.v. to inhibit NO synthase. After the first L-NAME dose diastolic blood pressure increased from 131.8+/-2.0 to 149.4+/-3.9 mm Hg (p<0.001) and was maintained at about this level till the end of the experiment. Systolic blood pressure only increased after the first dose (from 150.8+/-1.1 to 175.0+/-5.8 mm Hg, p<0.01), returning thereafter to the control level. Similarly, the heart rate declined only after the first dose (from 190.4+/-5.3 to 147.6+/-4.5 beats/min, p<0.01). Total RNA concentrations increased in the left cardiac ventricle (LV), the left anterior descending coronary artery (LADCA) and left circumflex coronary artery (LCCA) by 15.9+/-0.7, 29.7+/-1.3 and 17.6+/-1.0%, p<0.05, respectively. The same applied to [14C]leucine incorporation (by 86.5+/-5.0, 33.5+/-2.6, 29.3+/-4.1%, p<0.05, respectively). The above parameters indicated an increase of proteosynthesis in the LV myocardium and both coronary arteries LADCA and LCCA after short-term NO deficiency. Surprisingly, the ornithine decarboxylase activity in the LV myocardium decreased significantly by 40.2+/-1.6% (p<0.01) but the changes were not significant in the coronary arteries. This unexpected finding makes the role of polyamines in increasing proteosynthesis during a pressure overload due to NO deficiency questionable. |
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Authors:
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M Gerová; O Pechánová; I Bernátová; M Kittová; M Juráni |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: 47 ISSN: 0862-8408 ISO Abbreviation: Physiol Res Publication Date: 1998 |
Date Detail:
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Created Date: 1999-09-16 Completed Date: 1999-09-16 Revised Date: 2008-04-02 |
Medline Journal Info:
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Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: CZECH REPUBLIC |
Other Details:
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Languages: eng Pagination: 405-12 Citation Subset: IM |
Affiliation:
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Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Ivanka pri Dunaji, Slovak Republic. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arteries / metabolism Blood Pressure / drug effects Coronary Vessels / metabolism* Dogs Enzyme Inhibitors / pharmacology* Female Heart Rate / drug effects Leucine / metabolism Male Myocardium / metabolism* NG-Nitroarginine Methyl Ester / pharmacology* Nitric Oxide Synthase / antagonists & inhibitors* Nucleic Acids / metabolism Ornithine Decarboxylase / metabolism Protein Biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Nucleic Acids; 50903-99-6/NG-Nitroarginine Methyl Ester; 61-90-5/Leucine; EC 1.14.13.39/Nitric Oxide Synthase; EC 4.1.1.17/Ornithine Decarboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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