Document Detail

Early changes in lung tissue hyaluronan (hyaluronic acid) and hyaluronidase in bleomycin-induced alveolitis in hamsters.
MedLine Citation:
PMID:  1703735     Owner:  NLM     Status:  MEDLINE    
Intratracheal instillation of bleomycin in hamsters initiates a series of events that mimic human interstitial pulmonary fibrosis. Because glycosaminoglycans and particularly hyaluronan (hyaluronic acid, HA), may play an important role in the extracellular matrix response to early injury and subsequent fibrosis, this study was undertaken to define the early time course of changes in HA and hyaluronidase. Hamsters were given either 1 unit bleomycin sulfate in 0.2 ml saline or 0.2 ml saline (control), and randomly selected animals from both groups were killed at Days 3, 5, 6, 7, 9, and 17. Glycosaminoglycan fractions prepared from lung tissue of individual animals were analyzed for HA. The maximal HA content was reached 6 days after instillation of bleomycin and was 14.6-fold the normal value. The weight of injured lungs was 2.3-fold the control value. Thus, the increase in HA content was 30-fold. By Day 7 the HA content had dropped sharply. It then declined gradually to approximately double control values at Day 17. The specific activity of lysosomal hyaluronidase was the same in bleomycin-treated lungs and control lungs. Total units of the enzyme were increased in injured lungs, even at the time of maximal HA content, indicating active turnover of HA. The maximal HA content occurs prior to the rise in collagen and elastin biosynthesis. This observation in addition to the magnitude of the increase and its abrupt decline suggest that HA may be an important initiating factor for pathologic changes in lung extracellular matrix components.
B A Bray; P M Sampson; M Osman; A Giandomenico; G M Turino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  143     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1991 Feb 
Date Detail:
Created Date:  1991-03-07     Completed Date:  1991-03-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  284-8     Citation Subset:  AIM; IM    
St. Luke's-Roosevelt Hospital Center, New York, NY 10019.
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MeSH Terms
Chondroitin Sulfates / metabolism
Dermatan Sulfate / metabolism
Hyaluronic Acid / metabolism*
Hyaluronoglucosaminidase / metabolism*
Lung / metabolism*,  pathology
Organ Size
Pneumonia / chemically induced,  metabolism*,  pathology
Pulmonary Alveoli*
Time Factors
Grant Support
Reg. No./Substance:
11056-06-7/Bleomycin; 24967-94-0/Dermatan Sulfate; 9004-61-9/Hyaluronic Acid; 9007-28-7/Chondroitin Sulfates; EC

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