Document Detail


Early cellular responses in vitro to endotoxin administration.
MedLine Citation:
PMID:  6269776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sequence of early events which follow the administration of E coli lipopolysaccharide (LPS) to cultured mouse neuroblastoma (C-1300) cells was investigated. Emphasis was placed on cellular energy metabolism in order to establish whether or not an energy failure occurred and whether it was a primary or a secondary effect. Exposure of cultured neuroblastoma cells to LPS produced rapid changes in the regulatory parameters of energy metabolism, an oxidation of intramitochondrial pyridine nucleotides, and a decline in cellular [ATP]/[ADP] [Pi], which were followed by alterations in mitochondrial morphology. In spite of the changes in individual parameters at early stages of exposure to LPS, the cellular energy producing systems remained tightly controlled and the rate of ATP synthesis was maintained at a constant and undiminished level. This allowed the cells to preserve their ionic gradients as manifested by high intracellular [K+] and unaltered transmembrane electrical and pH gradients. These early changes in mitochondrial metabolism were not accompanied by detectable leakage of mitochondrial matrix enzymes into the cytosol, which indicated that mitochondrial membrane remained intact. After longer exposure to LPS, the rate of ATP synthesis declined, the mitochondrial membrane became permeable to high molecular weight substances (matrix enzymes), and intracellular [K+] began to decrease (K+ leakage). It was concluded that responses of mitochondrial metabolism are one of the early events in endotoxemia.
Authors:
L Kilpatrich-Smith; M Erecinska; I A Silver
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulatory shock     Volume:  8     ISSN:  0092-6213     ISO Abbreviation:  Circ. Shock     Publication Date:  1981  
Date Detail:
Created Date:  1981-12-21     Completed Date:  1981-12-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0414112     Medline TA:  Circ Shock     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  585-600     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / metabolism
Adenosine Triphosphate / metabolism
Animals
Cell Membrane / metabolism
Cells, Cultured
Cytochrome c Group / metabolism
Endotoxins / pharmacology*
Energy Metabolism
Glycolysis / drug effects
Lipopolysaccharides / pharmacology*
Mice
Mice, Inbred Strains
Mitochondria / metabolism
Neuroblastoma / metabolism*
Oxidation-Reduction / drug effects
Oxygen / metabolism
Time Factors
Grant Support
ID/Acronym/Agency:
GM 12202/GM/NIGMS NIH HHS; GM 21524/GM/NIGMS NIH HHS; NS 10939/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Cytochrome c Group; 0/Endotoxins; 0/Lipopolysaccharides; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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