| Early cell kinetic effects of a single dose of monochromatic ultraviolet B irradiation on hairless mouse epidermis. | |
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MedLine Citation:
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PMID: 3192954 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hairless mice were exposed to a single erythemic (25 mJ/cm2) or suberythemic dose (12.5 mJ/cm2) of ultraviolet B (UVB) irradiation at 297 nm. The cell kinetic changes were observed at several times during the first 7 d after the irradiation. The mitotic count, the mitotic rate (stathmokinetic method), and the number of suprabasal and basal cells were scored in histologic sections. The incorporation of [3H]thymidine was measured after pulse labeling, and the DNA distribution pattern was studied by flow cytometry. Initially, both UVB-doses induced a block or delay in the cell proliferation. The rate of entrance of cells into mitosis and the uptake of [3H]thymidine were reduced, and cells accumulated in the S phase of the cell cycle. Hence, during the first period after irradiation, UVB seemed to interfere with the DNA synthesis by inducing a prolonged S phase duration. The DNA synthesis rate was reduced to the same degree after both UVB-doses. From 24 h after irradiation rapid regenerative proliferation took place, most pronounced after the highest UVB-dose. Waves of proliferation seemed to arise from partially synchronized cohorts of cells proceeding through the cell cycle at a higher speed than normal. Thus, the present study indicates that UVB irradiation is comparable with the cell kinetic effects following both chemical skin carcinogens and non-carcinogenic skin irritants. UVB induces an inhibitory effect on the DNA synthesis activity, in addition to regenerative cell proliferation subsequent to cell toxicity. |
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Authors:
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W M Olsen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of investigative dermatology Volume: 91 ISSN: 0022-202X ISO Abbreviation: J. Invest. Dermatol. Publication Date: 1988 Dec |
Date Detail:
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Created Date: 1989-01-11 Completed Date: 1989-01-11 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0426720 Medline TA: J Invest Dermatol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 585-9 Citation Subset: IM |
Affiliation:
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Institute of Pathology, University of Oslo, Norway. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement Epidermis / cytology, radiation effects* Female Kinetics Male Mice Mice, Hairless Ultraviolet Rays |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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