| Early axonal and glial pathology in fetal sheep brains with leukomalacia induced by repeated umbilical cord occlusion. | |
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MedLine Citation:
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PMID: 10392747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We conducted a chronic preparation experiment involving near term fetal sheep to evaluate the contribution of umbilical cord occlusion to fetal brain injury. In experimental groups (n = 11), complete cord occlusion for 3 min followed by 5 min release, repeated 5 times were performed at 3 days after initial surgery. Instrumental cases without cord occlusion (n = 3) and uninstrumental twins (n = 6) were also examined as controls. Multiple necrotic foci predominantly in the periventricular white matter were found in the fetal brains examined at 1-3 days after cord occlusion. To estimate the contribution of early axonal and glial reaction to brain injury the following immunohistochemical study was performed. In the lesions, coagulation necrosis, axonal swelling and microglial activation were demonstrated with amyloid precursor protein or ionized calcium binding adapter molecule 1 immunohistochemistry. The induction of tumor necrosis factor alpha and inducible nitric oxide synthase were also detected immunohistochemically in the microglia at 1 and 3 days after cord occlusion. In contrast, the reaction of glial fibrillary acidic protein positive astrocytes was faint at 1 day after occlusion, but the induction of cyclooxygenase-2 was observed. These findings suggest the glial reaction of cytokines and free radicals induced by fetal hypoxia may contribute to the occurrence of brain injury. |
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Authors:
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J Ohyu; G Marumo; H Ozawa; S Takashima; K Nakajima; S Kohsaka; Y Hamai; Y Machida; K Kobayashi; E Ryo; K Baba; S Kozuma; T Okai; Y Taketani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain & development Volume: 21 ISSN: 0387-7604 ISO Abbreviation: Brain Dev. Publication Date: 1999 Jun |
Date Detail:
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Created Date: 1999-09-08 Completed Date: 1999-09-08 Revised Date: 2011-10-27 |
Medline Journal Info:
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Nlm Unique ID: 7909235 Medline TA: Brain Dev Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 248-52 Citation Subset: IM |
Affiliation:
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Department of Mental Retardation and Birth Defect Research, National Institute of Neuroscience, NCNP, Kodaira, Tokyo, Japan. ohyu@ncnaxp.ncnp.go.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid beta-Protein Precursor
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metabolism Animals Astrocytes / enzymology, metabolism Axons / pathology* Brain / metabolism, pathology* Calcium-Binding Proteins / metabolism Cerebral Cortex / metabolism, pathology Cyclooxygenase 2 DNA-Binding Proteins* Glial Fibrillary Acidic Protein / metabolism Humans Immunohistochemistry Infant, Newborn Isoenzymes / metabolism Leukomalacia, Periventricular / metabolism, pathology* Membrane Proteins Microglia / enzymology, metabolism Necrosis Neuroglia / metabolism, pathology* Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type II Prostaglandin-Endoperoxide Synthases / metabolism Sheep Thalamus / metabolism, pathology Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
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0/AIF1 protein, human; 0/Amyloid beta-Protein Precursor; 0/Calcium-Binding Proteins; 0/DNA-Binding Proteins; 0/Glial Fibrillary Acidic Protein; 0/Isoenzymes; 0/Membrane Proteins; 0/Tumor Necrosis Factor-alpha; EC 1.14.13.39/NOS2 protein, human; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/PTGS2 protein, human; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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