| Early alteration in leukocyte populations and Th1/Th2 function in ethanol-consuming mice. | |
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MedLine Citation:
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PMID: 11505054 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Chronic alcohol consumption polarizes the immune response away from Th1-mediated cell-mediated immunity. In the present report we investigate the first onset of alteration in immune parameters during ethanol consumption in terms of changes in splenic leukocyte cellularity and surface phenotype as well as alterations in Th1 and Th2 function. METHODS: BALB/c and C57BL/6 mice were fed ethanol-containing liquid diets, were pair-fed an isocaloric liquid control diet, or were fed solid diet and water ad libitum for up to 12 days. At intervals during the feeding period, splenic leukocytes were assessed for phenotypic markers by flow cytometry and for their ability to support antigen-induced interferon-gamma (IFNgamma) production in a coculture system. Mice were bled at intervals throughout the feeding period, and serum immunoglobin E (IgE) and alcohol levels were determined. RESULTS: Data show that phenotypic and functional alterations occur within the first few days of alcohol consumption. Both liquid diets affect splenic cellularity, and by dietary day 5, ethanol-containing liquid diets further reduce B and NK cell numbers. The decline in B cells is accompanied by a concomitant decline in the amount of major histocompatibility complex class II expressed on this population. Functional alteration in Th1-mediated IFNgamma production occurred in the population fed ethanol-containing liquid diets by dietary day 5. Th2 function, as indicated by systemic serum IgE levels in these unimmunized mice, is increased by dietary day 6 to 8 and correlated with significant blood alcohol levels. CONCLUSIONS: Ethanol consumption by mice causes a rapid decrease in splenic cellularity accompanied by a decrease in Th1 function and a rapid increase in systemic IgE levels. |
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Authors:
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S Starkenburg; M E Munroe; C Waltenbaugh |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Alcoholism, clinical and experimental research Volume: 25 ISSN: 0145-6008 ISO Abbreviation: Alcohol. Clin. Exp. Res. Publication Date: 2001 Aug |
Date Detail:
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Created Date: 2001-08-15 Completed Date: 2001-09-27 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7707242 Medline TA: Alcohol Clin Exp Res Country: United States |
Other Details:
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Languages: eng Pagination: 1221-30 Citation Subset: IM |
Affiliation:
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Department of Microbiology-Immunology, Northwestern University School of Medicine, Chicago, Illinois 60611-3073, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcoholism
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immunology Animals B-Lymphocytes Coculture Techniques Ethanol / administration & dosage* Female Histocompatibility Antigens Class II / analysis Immunoglobulin E / blood Interferon-gamma / biosynthesis Killer Cells, Natural Leukocyte Count* Lymphocyte Count Mice Mice, Inbred BALB C Mice, Inbred C57BL Self Administration Spleen / cytology T-Lymphocytes, Helper-Inducer / immunology* |
| Grant Support | |
ID/Acronym/Agency:
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AA05527/AA/NIAAA NIH HHS; AA08275/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Histocompatibility Antigens Class II; 37341-29-0/Immunoglobulin E; 64-17-5/Ethanol; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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