Document Detail

Early activation of transcription factor expression in Schwann cells by progesterone.
MedLine Citation:
PMID:  11750070     Owner:  NLM     Status:  MEDLINE    
Progesterone (PROG) promotes the myelination of sciatic nerves during regeneration after cryolesion. But, little is known about the molecular mechanisms by which the hormone exerts its effects. This could be initiated by the regulation of transcription factor expression in Schwann cells, which produce the myelin sheaths in the peripheral nervous system. We investigated by RT-PCR whether PROG activated expression of transcription factors: Egr-1 (Krox-24) Egr-2 (Krox-20), Egr-3, c-jun, jun B, jun D, c-Fos, Fos B, Fra-1, Fra-2, CREB, ATF 4, SCIP and Sox-10 in cultured Schwann cells. PROG triggered a quick (visible as soon as 15 min), strong (6 to 18-fold) and transient (1-2 h) stimulation of Egr-1, Egr-2, Egr-3 and Fos B genes expression. Expression of other genes remained unaffected by PROG treatment. The same expression pattern was obtained in the MSC 80 line (mouse Schwann cells), but not in the NIH-3T3 and CHO lines. Estradiol and testosterone induced different patterns of transcription factor gene activation in Schwann cells. Serum stimulated all genes activated by PROG in addition c-fos, fra-1 and fra-2. The PROG effects were blocked by Actinomycin D and by RU 486. This suggests that the activation of these genes occurs at the transcriptional level via the interaction of the hormone with its cognate receptor. Thus, PROG can regulate Schwann cell functions and differentiation by transiently activating specific transcription factors.
G Mercier; N Turque; M Schumacher
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  97     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-25     Completed Date:  2002-03-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  137-48     Citation Subset:  IM    
U 488 Inserm, 80 rue du Général Leclerc, 94276 Kremlin-Bicêtre, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
3T3 Cells
Activating Transcription Factor 4
CHO Cells
Cyclic AMP Response Element-Binding Protein / genetics
DNA-Binding Proteins / genetics
Dactinomycin / pharmacology
Early Growth Response Protein 1
Early Growth Response Protein 2
Early Growth Response Protein 3
Fos-Related Antigen-2
Gene Expression / drug effects,  physiology
Genes, Immediate-Early / physiology
High Mobility Group Proteins / genetics
Hormone Antagonists / pharmacology
Immediate-Early Proteins*
Mifepristone / pharmacology
Nucleic Acid Synthesis Inhibitors / pharmacology
Octamer Transcription Factor-6
Progesterone / pharmacology*
Proto-Oncogene Proteins c-fos / genetics
Proto-Oncogene Proteins c-jun / genetics
Rats, Sprague-Dawley
SOXE Transcription Factors
Schwann Cells / physiology*
Transcription Factors / genetics*
Reg. No./Substance:
0/Atf4 protein, rat; 0/Cyclic AMP Response Element-Binding Protein; 0/DNA-Binding Proteins; 0/Early Growth Response Protein 1; 0/Early Growth Response Protein 2; 0/Egr1 protein, mouse; 0/Egr1 protein, rat; 0/Egr2 protein, mouse; 0/Egr2 protein, rat; 0/Egr3 protein, mouse; 0/Egr3 protein, rat; 0/Fos-Related Antigen-2; 0/Fosl2 protein, mouse; 0/Fosl2 protein, rat; 0/High Mobility Group Proteins; 0/Hormone Antagonists; 0/Immediate-Early Proteins; 0/Nucleic Acid Synthesis Inhibitors; 0/Pou3f1 protein, mouse; 0/Pou3f1 protein, rat; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/SOXE Transcription Factors; 0/Sox10 protein, mouse; 0/Sox10 protein, rat; 0/Transcription Factors; 0/fos-related antigen 1; 132052-52-9/Octamer Transcription Factor-6; 144516-98-3/Early Growth Response Protein 3; 145891-90-3/Activating Transcription Factor 4; 50-76-0/Dactinomycin; 57-83-0/Progesterone; 84371-65-3/Mifepristone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Stimulation of gene expression of NeuroD-related factor in the mouse brain following pentylenetetraz...
Next Document:  Neuronal promoter of human aromatic L-amino acid decarboxylase gene directs transgene expression to ...