| Early activation and cell cycle entry of resting B cells after Fab-anti-Ig treatment: role of receptor crosslinking. | |
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MedLine Citation:
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PMID: 2463097 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Comparison of the effect of goat anti-rabbit Ig (GARIg) and its monovalent fragment (Fab-GARIg) demonstrates that surface Ig (sIg) crosslinking is not necessary to effect G0 to G1 transition in rabbit peripheral blood B cells but is required for induction of DNA synthesis. Five micrograms per milliliter or more of GARIg is sufficient to induce DNA synthesis but up to 50 micrograms/ml of Fab-GARIg is not. However, the monovalent reagent induces microscopically observable cytoplasmic and nuclear changes (blast transformation) in a dose-dependent manner. These differ qualitatively and quantitatively from the morphological changes seen with comparable doses of GARIg; Fab anti-Ig produces "small blasts" whereas complete GARIg induces large blasts. The monovalent reagent, in a wide range of concentrations, is as effective as the complete antibody in modulating sIg from rabbit B cells. Fab-GARIg treatment modulates sIg in a biphasic manner. It clears the high-density sIg within 5 min, whereas the remaining low-density receptors disappear after 4 hr. Cytosolic protein kinase C levels decline equally after treatment with either Fab-GARIg or whole anti-Ig. RNA synthesis, as measured by [3H]uridine incorporation, increases for the first 12 hr in cells activated with either reagent. It declines to basal levels in Fab-GARIg stimulated cells, but a continuous increase occurs in cells stimulated with 5 and 50 micrograms/ml of complete antibody. Simultaneous addition of 50 micrograms/ml Fab-GARIg with 5 microgram/ml of GARIg causes greater RNA synthesis for 12 hr after stimulation than is caused by GARIg alone. After 12 hr the monovalent reagent has an inhibitory effect on RNA synthesis. Fluorescence-activated cell sorter analysis of acridine orange-stained cells shows that Fab anti-Ig-stimulated cells have higher RNA content than resting cells, but lower than GARIg-activated cells. These findings suggest that rabbit B cells can be activated from the G0 stage of cell cycle to G1 by monovalent anti-Ig reagents but further cell cycle progression requires maintenance signals provided by receptor crosslinking. The implications of these results for B cell activation signalling are discussed in the context of the floating receptor model. |
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Authors:
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A Lagoo; S Sell |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cellular immunology Volume: 118 ISSN: 0008-8749 ISO Abbreviation: Cell. Immunol. Publication Date: 1989 Jan |
Date Detail:
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Created Date: 1989-02-16 Completed Date: 1989-02-16 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1246405 Medline TA: Cell Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 53-67 Citation Subset: IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston 77025. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Anti-Idiotypic / immunology* B-Lymphocytes / immunology* Cell Cycle DNA Replication Immunoglobulin Fab Fragments / immunology* Lymphocyte Activation* Male Protein Binding Protein Kinase C / analysis RNA / biosynthesis Rabbits Receptors, Antigen, B-Cell / immunology* Receptors, Fc / immunology* Signal Transduction |
| Grant Support | |
ID/Acronym/Agency:
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A121290-01//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Anti-Idiotypic; 0/Immunoglobulin Fab Fragments; 0/Receptors, Antigen, B-Cell; 0/Receptors, Fc; 63231-63-0/RNA; EC 2.7.11.13/Protein Kinase C |
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