Document Detail

Early activation and cell cycle entry of resting B cells after Fab-anti-Ig treatment: role of receptor crosslinking.
MedLine Citation:
PMID:  2463097     Owner:  NLM     Status:  MEDLINE    
Comparison of the effect of goat anti-rabbit Ig (GARIg) and its monovalent fragment (Fab-GARIg) demonstrates that surface Ig (sIg) crosslinking is not necessary to effect G0 to G1 transition in rabbit peripheral blood B cells but is required for induction of DNA synthesis. Five micrograms per milliliter or more of GARIg is sufficient to induce DNA synthesis but up to 50 micrograms/ml of Fab-GARIg is not. However, the monovalent reagent induces microscopically observable cytoplasmic and nuclear changes (blast transformation) in a dose-dependent manner. These differ qualitatively and quantitatively from the morphological changes seen with comparable doses of GARIg; Fab anti-Ig produces "small blasts" whereas complete GARIg induces large blasts. The monovalent reagent, in a wide range of concentrations, is as effective as the complete antibody in modulating sIg from rabbit B cells. Fab-GARIg treatment modulates sIg in a biphasic manner. It clears the high-density sIg within 5 min, whereas the remaining low-density receptors disappear after 4 hr. Cytosolic protein kinase C levels decline equally after treatment with either Fab-GARIg or whole anti-Ig. RNA synthesis, as measured by [3H]uridine incorporation, increases for the first 12 hr in cells activated with either reagent. It declines to basal levels in Fab-GARIg stimulated cells, but a continuous increase occurs in cells stimulated with 5 and 50 micrograms/ml of complete antibody. Simultaneous addition of 50 micrograms/ml Fab-GARIg with 5 microgram/ml of GARIg causes greater RNA synthesis for 12 hr after stimulation than is caused by GARIg alone. After 12 hr the monovalent reagent has an inhibitory effect on RNA synthesis. Fluorescence-activated cell sorter analysis of acridine orange-stained cells shows that Fab anti-Ig-stimulated cells have higher RNA content than resting cells, but lower than GARIg-activated cells. These findings suggest that rabbit B cells can be activated from the G0 stage of cell cycle to G1 by monovalent anti-Ig reagents but further cell cycle progression requires maintenance signals provided by receptor crosslinking. The implications of these results for B cell activation signalling are discussed in the context of the floating receptor model.
A Lagoo; S Sell
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular immunology     Volume:  118     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-02-16     Completed Date:  1989-02-16     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  53-67     Citation Subset:  IM    
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, Houston 77025.
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MeSH Terms
Antibodies, Anti-Idiotypic / immunology*
B-Lymphocytes / immunology*
Cell Cycle
DNA Replication
Immunoglobulin Fab Fragments / immunology*
Lymphocyte Activation*
Protein Binding
Protein Kinase C / analysis
RNA / biosynthesis
Receptors, Antigen, B-Cell / immunology*
Receptors, Fc / immunology*
Signal Transduction
Grant Support
A121290-01//PHS HHS
Reg. No./Substance:
0/Antibodies, Anti-Idiotypic; 0/Immunoglobulin Fab Fragments; 0/Receptors, Antigen, B-Cell; 0/Receptors, Fc; 63231-63-0/RNA; EC Kinase C

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