Document Detail


Early tissue transglutaminase-mediated response underlies K562(S)-cell gliadin-dependent agglutination.
MedLine Citation:
PMID:  22314661     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTODUCTION: K562(S) agglutination has been used as a rapid and economic tool for the in vitro screening of the toxicity of cereal fractions and prolamins in celiac disease (CD). A strict correlation has been reported between the toxicity of cereals and cereal fractions for celiac patients and their ability to agglutinate K562(S) cells. Whether this specificity of K562(S)-cell agglutination is caused by the activation of the same pathogenic events triggered by toxic cereal fractions in CD intestine or simply represents a bystander event of gluten toxicity is, however, unknown.
METHODS: K562(S) cells were incubated in vitro with the peptic-tryptic digest of wheat gliadin.
RESULTS: The agglutination of K562(S) cells by wheat gliadin peptides is orchestrated by a cascade of very early events occurring at the K562(S)-cell surface similar to those occurring at the intestinal epithelial surface. They involve a rapid increase in intracellular calcium levels that activate tissue transglutaminase (TG2), leading to a rapid actin reorganization that is pivotal in driving cell agglutination. These specific effects of toxic cereals are phenocopied by the gliadin-derived peptide p31-43, which orchestrates the activation of innate response to gliadin in CD.
DISCUSSION: Our study provides the rationale for the extensive use of K562(S)-cell agglutination as a valuable tool for screening cereal toxicity.
Authors:
Marco Silano; Olimpia Vincentini; Alessandro Luciani; Cristina Felli; Sergio Caserta; Speranza Esposito; Valeria Rachela Villella; Massimo Pettoello-Mantovani; Stefano Guido; Luigi Maiuri
Publication Detail:
Type:  Journal Article     Date:  2012-02-07
Journal Detail:
Title:  Pediatric research     Volume:  71     ISSN:  1530-0447     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-18     Completed Date:  2012-08-06     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  532-8     Citation Subset:  IM    
Affiliation:
Unit of Human Nutrition and Health, Istituto Superiore di Sanità, Rome, Italy. marco.silano@iss.it
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MeSH Terms
Descriptor/Qualifier:
Cell Adhesion*
Enzyme Activation
Gliadin / metabolism*
Humans
K562 Cells
Transglutaminases / metabolism*
Chemical
Reg. No./Substance:
9007-90-3/Gliadin; EC 2.3.2.13/Transglutaminases; EC 2.3.2.13/tissue transglutaminase 2, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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