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Early Onset of Endothelial cell Proliferation in Coronary Thrombi of Patients with Acute Myocardial Infarction - Implications for Plaque Healing.
MedLine Citation:
PMID:  22235978     Owner:  NLM     Status:  Publisher    
Aims: Coronary thrombotic occlusion in ST-segment elevation myocardial infarction (STEMI) patients is often preceded by episodes of progressive growth of the thrombus mass. Similar to wound healing, the organization of thrombus could depend on ingrowth of microvessels in order to stabilize its structure. We investigated the patterns of neovascularization in different stages of coronary thrombus evolution. Material and methods: Thrombectomy materials obtained from STEMI patients were histologically classified according to thrombus age in three groups: fresh (<1 day), lytic (1-5 days) or organized (>5 days) thrombi. Forty thrombi of each group were randomly collected. Neovascularization in the thrombi was evaluated histomorphologically and with immunodouble stains to visualize various differentiation antigens of endothelial cells (ECs) and primitive cells. Results: Morphologically, ECs in the coronary thrombi manifested as: single cells, cell clusters or microvessels. CD31+/CD34+ ECs were present in 98% of all the thrombi. In addition, endothelial clusters were found in 63% of the fresh thrombi (<1 day). CD105+, Ki67+, or C-kit+ ECs (active, proliferating cells) were observed in all the stages, but significantly more in organized thrombi (>5 days) compared with fresh and lytic ones (<5 days), and mainly as cell clusters (P≤0.05 for all). CD133+ primitive cells were found only sporadically in 11% of all the samples. Conclusion: EC proliferation is initiated very early, and gradually progresses during the organization process of thrombus after coronary plaque disruption, with only limited contribution of primitive cells in this process.
X Li; M C Kramer; C M van der Loos; H J P Ploegmakers; O J de Boer; K T Koch; J G P Tijssen; R J de Winter; A C van der Wal
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-11
Journal Detail:
Title:  Journal of thrombosis and haemostasis : JTH     Volume:  -     ISSN:  1538-7836     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101170508     Medline TA:  J Thromb Haemost     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 International Society on Thrombosis and Haemostasis.
Departments of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam Departments of Cardiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
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