Document Detail


Early Depletion of Primordial Germ Cells in Zebrafish Promotes Testis Formation.
MedLine Citation:
PMID:  25434820     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
As complete absence of germ cells leads to sterile males in zebrafish, we explored the relationship between primordial germ cell (PGC) number and sexual development. Our results revealed dimorphic proliferation of PGCs in the early zebrafish larvae, marking the beginning of sexual differentiation. We applied morpholino-based gene knockdown and cell transplantation strategies to demonstrate that a threshold number of PGCs is required for the stability of ovarian fate. Using histology and transcriptomic analyses, we determined that zebrafish gonads are in a meiotic ovarian stage at 14 days postfertilization and identified signaling pathways supporting meiotic oocyte differentiation and eventual female fate. The development of PGC-depleted gonads appears to be restrained and delayed, suggesting that PGC number may directly regulate the variability and length of gonadal transformation and testicular differentiation in zebrafish. We propose that gonadal transformation may function as a developmental buffering mechanism to ensure the reproductive outcome.
Authors:
Keh-Weei Tzung; Rie Goto; Jolly M Saju; Rajini Sreenivasan; Taiju Saito; Katsutoshi Arai; Etsuro Yamaha; Mohammad Sorowar Hossain; Meredith E K Calvert; László Orbán
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-25
Journal Detail:
Title:  Stem cell reports     Volume:  -     ISSN:  2213-6711     ISO Abbreviation:  Stem Cell Reports     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-12-1     Completed Date:  -     Revised Date:  2014-12-2    
Medline Journal Info:
Nlm Unique ID:  101611300     Medline TA:  Stem Cell Reports     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Zeta Inhibitory Peptide (ZIP) Erases Long-term Memories in a Cockroach.
Next Document:  Tracing Dynamics and Clonal Heterogeneity of Cbx7-Induced Leukemic Stem Cells by Cellular Barcoding.