Document Detail


Early assessment of pulmonary inflammation by 19F MRI in vivo.
MedLine Citation:
PMID:  20061515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Emulsified perfluorocarbons (PFCs) are preferentially phagocytized by monocytes/macrophages and are readily detected by (19)F MRI. This study tests the hypothesis that (19)F MRI can be used to quantitate pulmonary inflammation by tracking of infiltrating PFC-loaded monocytes.
METHODS AND RESULTS: Pneumonia was induced in mice by intratracheal instillation of lipopolysaccharides (LPS) followed by intravenous injection of PFCs. Whereas regular (1)H MRI provided no evidence of lung injury 24 hours after LPS, the concurrent (19)F images clearly show PFC accumulation in both pulmonary lobes. Imaging at 48 hours after LPS revealed signals in (1)H images at the same location as the 24-hour (19)F signals. Thus, progressive pneumonia was first predicted by (19)F MRI early after PFC administration. Without LPS, at no time were (19)F signals observed within the lung. Histology and fluorescence-activated cell sorting (FACS) combined with (19)F MRI confirmed the presence of infiltrating PFC-loaded monocytes/macrophages after LPS challenge. Additional experiments with graded doses of LPS demonstrated that (19)F signal intensity strongly correlated with both LPS dose and pathological markers of lung inflammation. In separate studies, dexamethasone and CGS21680 (adenosine 2A receptor agonist) were used to demonstrate the ability of (19)F MRI to monitor anti-inflammatory therapies.
CONCLUSIONS: PFCs serve as a contrast agent for the prognostic and quantitative assessment of pulmonary inflammation by in vivo (19)F MRI, which is characterized by a high degree of specificity due to the lack of any (19)F background. Because PFCs are biochemically inert, this approach may also be suitable for human applications.
Authors:
Bernd Ebner; Patrick Behm; Christoph Jacoby; Sandra Burghoff; Brent A French; Jürgen Schrader; Ulrich Flögel
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-01-08
Journal Detail:
Title:  Circulation. Cardiovascular imaging     Volume:  3     ISSN:  1942-0080     ISO Abbreviation:  Circ Cardiovasc Imaging     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-05-07     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  101479935     Medline TA:  Circ Cardiovasc Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  202-10     Citation Subset:  IM    
Affiliation:
Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität, Düsseldorf, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Disease Models, Animal
Emulsions
Flow Cytometry
Fluorine / diagnostic use
Fluorocarbons / diagnostic use
Immunohistochemistry
Lipopolysaccharides
Magnetic Resonance Imaging / methods*
Mice
Monocytes
Pneumonia / diagnosis*
Grant Support
ID/Acronym/Agency:
P01 HL073361/HL/NHLBI NIH HHS; P01 HL073361-04/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Emulsions; 0/Fluorocarbons; 0/Lipopolysaccharides; 7782-41-4/Fluorine
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