| EZ Gluten for the qualitative detection of gluten in foods, beverages, and environmental surfaces. | |
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MedLine Citation:
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PMID: 22970579 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The EZ Gluten assay is a rapid immunochromatographic screening method for qualitative detection of gluten in raw and cooked foods and beverages and on environmental surfaces. This AOAC Performance Tested Method study evaluated the EZ Gluten assay as an effective method for the detection of gluten in four selected matrixes: rice flour, cooked dough, beer, and dog food. In addition, the method was evaluated for its effectiveness in detecting gluten contamination of > or =1 microg/2 in.2 (25 cm2) stainless steel surface area. The EZ Gluten demonstrated 100% specificity [probability of detection (POD) 0.00, confidence interval (CI) 0.00-0.01] and 99% sensitivity (POD 0.99, CI 0.97-0.995) at the 10 ppm level for all four matrixes, and 100% specificity (POD 0.00, CI 0.00-0.11) and sensitivity (POD 1.00, CI 0.886-1.00) at the 1 microg level on the stainless steel surface. Independent laboratory testing confirmed the internal validation results in one matrix and on the stainless steel surface. Lot-to-lot, stability, and robustness studies provided evidence that the EZ Gluten is a rugged, consistent method for the detection of gluten at levels as low as 10 ppm. |
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Authors:
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Laura K Allred; Eun S Park |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of AOAC International Volume: 95 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2012 Jul-Aug |
Date Detail:
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Created Date: 2012-09-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
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Languages: eng Pagination: 1106-17 Citation Subset: IM |
Affiliation:
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ELISA Technologies, Inc., 2501 NW 66th Ct, Gainesville, FL 32653, USA. laura.allred@elisa-tek.com |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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