Document Detail

ET-1 induces pancreatitis-like microvascular deterioration and acinar cell injury.
MedLine Citation:
PMID:  10423333     Owner:  NLM     Status:  MEDLINE    
Using in vivo microscopy red blood cell (RBC) velocities, functional capillary density (FCD) and capillary diameters were estimated after inducing acute pancreatitis by intraductal infusion of sodium taurocholate (0.8 ml; 4%) or after topical superfusion of the pancreas with ET-1 (100 pmol). Sodium taurocholate mediated a significant decrease in RBC velocities between 50 and 70%, transient decrease in capillary diameters by 10%, and a sustained decrease in FCD between 60 and 70% paralleled by a dramatic heterogeneity in blood flow. Topical superfusion of the exteriorized pancreas with ET-1 caused a significant decrease in RBC velocities between 65 and 75%, a sustained decrease in capillary diameters by 10%, and a decrease in FCD by 45% accompanied by an increase in flow heterogeneity. Following sodium taurocholate infusion pancreas histology revealed a severe edema and sublobular acinar cell necrosis, while topical ET-1 application displayed a severe edema of the pancreas with focal acinar cell necrosis. Thus, ET-1 mediated a deterioration of the pancreatic microcirculation, which is similar to the microcirculatory failure found in sodium taurocholate-induced experimental pancreatitis and was associated with focal acinar cell necrosis. We are thus inclined to hypothesize that endothelin released by injured endothelial cells during acute biliary pancreatitis promotes microcirculatory failure and ischemia in acute pancreatitis, eventually leading to acinar cell necrosis.
T Plusczyk; B Bersal; S Westermann; M Menger; G Feifel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  85     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-08-30     Completed Date:  1999-08-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  301-10     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Department of General Surgery, University of Saarland, Homburg/Saar, 66421, Germany.
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MeSH Terms
Acute Disease
Administration, Topical
Blood Flow Velocity / drug effects
Capillaries / drug effects
Endothelin-1 / administration & dosage,  pharmacology*
Microcirculation / drug effects*
Pancreas / blood supply,  drug effects*,  pathology
Pancreatitis / chemically induced,  physiopathology*
Rats, Inbred Lew
Regional Blood Flow / drug effects
Taurocholic Acid / pharmacology
Reg. No./Substance:
0/Endothelin-1; 81-24-3/Taurocholic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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