| An ESR contrast agent is transported to rat liver through organic anion transporter. | |
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MedLine Citation:
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PMID: 16517505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Carboxy PROXYL is a useful extracellular paramagnetic contrast reagent in electron spin resonance (ESR) and magnetic resonance imaging (MRI). Active transfer of the probe was investigated using an in situ liver model in rats. Carboxy PROXYL, a nitroxyl spin probe, was perfused into in situ liver perfusion system from Wistar rats. Concentration of nitroxyl form of the spin probe in effluent increased gradually after introducing perfusate with the spin probe and reached a plateau. The disappearance of Carboxy PROXYL from the perfusate was 40%, which could not be explained with its partition coefficient. Administration of non-selective inhibitors of organic anion transporters, p-aminohippuric acid and penicillin G, inhibited competitively and in a dose dependent manner the transfer of Carboxy PROXYL into rat liver in situ, resulting in increases of Carboxy PROXYL in the effluent. The results demonstrate that there is an active transfer system of an ESR contrast reagent into in situ rat liver through organic anion transporters. |
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Authors:
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Kazuhiro Ichikawa; Yasuko Sato; Hiroshi Kondo; Hideo Utsumi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Free radical research Volume: 40 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 403-8 Citation Subset: IM |
Affiliation:
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Department of Bio-function Analysis, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-Ku, Fukuoka 812-8582, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport, Active / physiology Contrast Media / metabolism*, pharmacokinetics* Electron Spin Resonance Spectroscopy* Liver / metabolism* Organic Anion Transporters / metabolism* Rats Spin Labels |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 0/Organic Anion Transporters; 0/Spin Labels |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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