| EPR oximetry: reduction of cell respiration by vinblastine. | |
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MedLine Citation:
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PMID: 8739364 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Influence of vinblastine (VLB) on the rate of oxygen uptake by HeLa cells was studied using the electron paramagnetic resonance (EPR) method. A suspension of cells was labelled with spin probe 15N PDT. Broadening of the EPR spectrum line widths in the presence of O2 was used to measure its concentration throughout the sample. VLB decreased the rate of O2 consumption, but did not influence the viability of cells at the concentration used (1 ng/ml). In addition, intracellular O2 concentration was measured using a paramagnetic broadening agent CrOX. No significant difference in the intracellular O2 concentration with respect to the extracellular was observed neither in VLB treated nor in untreated cells. |
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Authors:
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D Dolenc; M Sentjurc; G Sersa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Pflügers Archiv : European journal of physiology Volume: 431 ISSN: 0031-6768 ISO Abbreviation: Pflugers Arch. Publication Date: 1996 |
Date Detail:
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Created Date: 1997-01-16 Completed Date: 1997-01-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0154720 Medline TA: Pflugers Arch Country: GERMANY |
Other Details:
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Languages: eng Pagination: R261-2 Citation Subset: IM |
Affiliation:
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J. Stefan Institute, University of Ljubljana, Slovenia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antineoplastic Agents, Phytogenic
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pharmacology* Cell Respiration / drug effects* Depression, Chemical Electron Spin Resonance Spectroscopy Hela Cells Humans Oximetry / methods* Oxygen Consumption / drug effects Spin Labels Vinblastine / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents, Phytogenic; 0/Spin Labels; 865-21-4/Vinblastine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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