| EP₃ receptors mediate PGE₂-induced hypothalamic paraventricular nucleus excitation and sympathetic activation. | |
| | |
MedLine Citation:
|
PMID: 21803943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Prostaglandin E(2) (PGE(2)), an important mediator of the inflammatory response, acts centrally to elicit sympathetic excitation. PGE(2) acts on at least four E-class prostanoid (EP) receptors known as EP(1), EP(2), EP(3), and EP(4). Since PGE(2) production within the brain is ubiquitous, the different functions of PGE(2) depend on the expression of these prostanoid receptors in specific brain areas. The type(s) and location(s) of the EP receptors that mediate sympathetic responses to central PGE(2) remain unknown. We examined this question using PGE(2), the relatively selective EP receptor agonists misoprostol and sulprostone, and the available selective antagonists for EP(1), EP(3), and EP(4). In urethane-anesthetized rats, intracerebroventricular (ICV) administration of PGE(2), sulprostone or misoprostol increased renal sympathetic nerve activity, blood pressure, and heart rate. These responses were significantly reduced by ICV pretreatment with the EP(3) receptor antagonist; the EP(1) and EP(4) receptor antagonists had little or no effect. ICV PGE(2) or misoprostol increased the discharge of neurons in the hypothalamic paraventricular nucleus (PVN). ICV misoprostol increased the c-Fos immunoreactivity of PVN neurons, an effect that was substantially reduced by the EP(3) receptor antagonist. Real-time PCR detected EP(3) receptor mRNA in PVN, and immunohistochemical studies revealed sparsely distributed EP(3) receptors localized in GABAergic terminals and on a few PVN neurons. Direct bilateral PVN microinjections of PGE(2) or sulprostone elicited sympathoexcitatory responses that were significantly reduced by the EP(3) receptor antagonist. These data suggest that EP(3) receptors mediate the central excitatory effects of PGE(2) on PVN neurons and sympathetic discharge. |
| | |
Authors:
|
Zhi-Hua Zhang; Yang Yu; Shun-Guang Wei; Yoshiko Nakamura; Kazuhiro Nakamura; Robert B Felder |
Related Documents
:
|
1668093 - Epidermal growth factor enhancement of insulin-like growth factor-i-induced down-regula... 8912113 - Wisc-iii and kait results in adolescent delinquent males. 7611703 - Roles of metabotropic glutamate receptors in brain plasticity and pathology. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-07-29 |
Journal Detail:
|
Title: American journal of physiology. Heart and circulatory physiology Volume: 301 ISSN: 1522-1539 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-09-29 Completed Date: 2011-11-22 Revised Date: 2013-02-08 |
Medline Journal Info:
|
Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
|
Languages: eng Pagination: H1559-69 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Pressure / drug effects Cerebrovascular Circulation / drug effects Cyclooxygenase 2 / biosynthesis, genetics Dinoprostone / administration & dosage, analogs & derivatives, pharmacology* Electrophysiological Phenomena Fluorescent Antibody Technique Heart Rate / drug effects Hemodynamics / drug effects Immunohistochemistry Injections, Intraventricular Kidney / drug effects, innervation Male Microcirculation / physiology Misoprostol / administration & dosage, pharmacology Paraventricular Hypothalamic Nucleus / blood supply, drug effects* Proto-Oncogene Proteins c-fos / biosynthesis Rats Rats, Sprague-Dawley Receptors, Prostaglandin E, EP3 Subtype / physiology* Reverse Transcriptase Polymerase Chain Reaction Stimulation, Chemical Sympathetic Nervous System / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 HL073986-05A2/HL/NHLBI NIH HHS; R01 HL073986-06/HL/NHLBI NIH HHS; R01 HL073986-07/HL/NHLBI NIH HHS; R01 HL073986-08/HL/NHLBI NIH HHS; R01-HL-073986/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Proto-Oncogene Proteins c-fos; 0/Receptors, Prostaglandin E, EP3 Subtype; 363-24-6/Dinoprostone; 59122-46-2/Misoprostol; 60325-46-4/sulprostone; EC 1.14.99.1/Cyclooxygenase 2 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: SEX HORMONE CONTROL OF LEFT VENTRICULAR STRUCTURE/FUNCTION: MECHANISTIC INSIGHTS USING ECHOCARDIOGRA...
Next Document: Apelin-13 protects the heart against ischemia-reperfusion injury through inhibition of ER-dependent ...