Document Detail


ENVOY is a major determinant in regulation of sexual development in Hypocrea jecorina (Trichoderma reesei).
MedLine Citation:
PMID:  22581525     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Light is one crucial environmental signal which can determine whether a fungus reproduces asexually or initiates sexual development. Mating in the ascomycete Hypocrea jecorina (anamorph Trichoderma reesei) occurs preferentially in light. We therefore investigated the relevance of the light response machinery for sexual development in H. jecorina. We found that the photoreceptors BLR1 and BLR2 and the light-regulatory protein ENV1 have no effect on male fertility, while ENV1 is essential for female fertility. BLR1 and BLR2 were found to impact fruiting body formation although they are not essential for mating. Quantitative reverse transcription-PCR (qRT-PCR) analyses revealed that BLR1, BLR2, and ENV1 negatively regulate transcript levels of both pheromone receptors as well as peptide pheromone precursors in light but not in darkness and in a mating type-dependent manner. The effect of BLR1 and BLR2 on regulation of pheromone precursor and receptor genes is less severe than that of ENV1 as strains lacking env1 show 100-fold (for ppg1) to more than 100,000-fold (for hpp1) increased transcript levels of pheromone precursor genes as well as more than 20-fold increased levels of hpr1, the pheromone receptor receiving the HPP1 signal in a MAT1-1 strain. ENV1 likely integrates additional signals besides light, and our results indicate that its function is partially mediated via regulation of mat1-2-1. We conclude that ENV1 is essential for balancing the levels of genes regulated in a mating-type-dependent manner, which contributes to determination of sexual identity and fruiting body formation.
Authors:
Christian Seibel; Doris Tisch; Christian P Kubicek; Monika Schmoll
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-11
Journal Detail:
Title:  Eukaryotic cell     Volume:  11     ISSN:  1535-9786     ISO Abbreviation:  Eukaryotic Cell     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-29     Completed Date:  2012-12-03     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  101130731     Medline TA:  Eukaryot Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  885-95     Citation Subset:  IM    
Affiliation:
Research Area Gene Technology and Applied Biochemistry, Institute of Chemical Engineering, Vienna University of Technology, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Fungal Proteins / genetics,  metabolism*
Gene Expression Regulation, Developmental* / radiation effects
Gene Expression Regulation, Fungal / radiation effects
Genes, Mating Type, Fungal / radiation effects
Light
Reproduction / radiation effects
Trichoderma / genetics,  growth & development,  physiology*,  radiation effects
Chemical
Reg. No./Substance:
0/Fungal Proteins
Comments/Corrections

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