Document Detail


ELOVL3 is an important component for early onset of lipid recruitment in brown adipose tissue.
MedLine Citation:
PMID:  16326704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During the recruitment process of brown adipose tissue, the mRNA level of the fatty acyl chain elongase Elovl3 is elevated more than 200-fold in cold-stressed mice. We have obtained Elovl3-ablated mice and report here that, although cold-acclimated Elovl3-ablated mice experienced an increased heat loss due to impaired skin barrier, they were unable to hyperrecruit their brown adipose tissue. Instead, they used muscle shivering in order to maintain body temperature. Lack of Elovl3 resulted in a transient decrease in the capacity to elongate saturated fatty acyl-CoAs into very long chain fatty acids, concomitantly with the occurrence of reduced levels of arachidic acid (C20:0) and behenic acid (C22:0) in brown adipose tissue during the initial cold stress. This effect on very long chain fatty acid synthesis could be illustrated as a decrease in the condensation activity of the elongation enzyme. In addition, warm-acclimated Elovl3-ablated mice showed diminished ability to accumulate fat and reduced metabolic capacity within the brown fat cells. This points to ELOVL3 as an important regulator of endogenous synthesis of saturated very long chain fatty acids and triglyceride formation in brown adipose tissue during the early phase of the tissue recruitment.
Authors:
Rolf Westerberg; Jan-Erik Månsson; Valeria Golozoubova; Irina G Shabalina; Emma C Backlund; Petr Tvrdik; Kjetil Retterstøl; Mario R Capecchi; Anders Jacobsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-02
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  281     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-05-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4958-68     Citation Subset:  IM    
Affiliation:
Wenner-Gren Institute, The Arrhenius Laboratories F3, Stockholm University, SE-106 91 Stockholm, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Acetyltransferases
Adipose Tissue / metabolism*
Adipose Tissue, Brown / metabolism
Animals
Body Temperature
Body Weight
Chromatography, Gas
Cold Temperature
Crosses, Genetic
Eicosanoic Acids / chemistry
Electromyography
Fatty Acids / chemistry,  metabolism
Female
Lipids / chemistry
Male
Membrane Proteins / genetics,  physiology*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Mitochondria / metabolism
Oxygen Consumption
RNA / metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Triglycerides / metabolism
Chemical
Reg. No./Substance:
0/Eicosanoic Acids; 0/Fatty Acids; 0/Lipids; 0/Membrane Proteins; 0/RNA, Messenger; 0/Triglycerides; 112-85-6/behenic acid; 506-30-9/arachidic acid; 63231-63-0/RNA; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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