Document Detail


ELISA kit for casein determination: interlaboratory study.
MedLine Citation:
PMID:  20480915     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An interlaboratory study was performed in eight laboratories to validate an ELISA method developed for quantitative determination of casein in foods. The ELISA kit used is based on rabbit polyclonal antibodies. The kit is quite specific; no false-positive results or cross-reactivities were obtained for a broad range of food matrixes with zero content of milk proteins. All participants in the study received the casein kit, which included a standard operating procedure, a list of the samples, the samples, and a protocol for recording test results. The study included nine food samples: wheat flour, buckwheat flour, instant potato pur?e with milk, instant coffee with sugar and cream, a mixture for fancy bread, salami, liver pat?, chocolate muesli with nuts, and a mixture for gluten-free bread. Three food samples with zero content of milk proteins showed a casein content lower than the lowest casein standard (1.0 mg CAS/kg) in most laboratories and measurements (64%). In 98% of the cases, the casein content was lower than the estimated LOQ. Two food samples with no dairy ingredient declared on the ingredient list contained casein levels higher than the second casein standard (3.0 mg CAS/kg) and the third standard (10.0 mg CAS/kg), respectively. Four food samples containing milk as an ingredient tested positive, and three showed casein contents higher than the highest standard (30.0 mg CAS/kg). The statistical tests (Cochran, Dixon) and analysis of variance were used for evaluation of the interlaboratory study results. Repeatability and reproducibility limits as well as LOQ (1.8 mg CAS/kg) and LOD (0.5 mg CAS/kg) for the kit were calculated from the results of the interlaboratory study.
Authors:
Frantisek Stumr; Dana Gabrovsk?; Jana Rysov?; Petr Han?k; Jan Plicka; Kv?ta Tomkov?; Pavla Dvorsk?; Petr Cuhra; Martin Kub?k; Sona Barsov?; Lenka Karsul?nov?; Hana Bulawov?; Josef Brychta; Ingrid Malmheden Yman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  93     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2010 Mar-Apr
Date Detail:
Created Date:  2010-05-19     Completed Date:  2010-06-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  676-82     Citation Subset:  IM    
Affiliation:
SEDIUM RD, Ltd, Zeleznicn?ho pluku 1361, 530 02 Pardubice, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Allergens / analysis
Animals
Calibration
Caseins / analysis*
Cross Reactions
Dairy Products / analysis
Enzyme-Linked Immunosorbent Assay / methods*
Food
Food Analysis / methods*
Milk Proteins / chemistry
Rabbits
Reference Standards
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Allergens; 0/Caseins; 0/Milk Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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