| EIF2alpha and caspase-12 activation are involved in oxygen-glucose-serum deprivation/restoration-induced apoptosis of spinal cord astrocytes. | |
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MedLine Citation:
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PMID: 20444431 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Astrocytes play an important role in protecting neurons during ischemia and reperfusion in the central nervous system. Although many studies have shown that oxygen-glucose deprivation (OGD) can induce astrocyte apoptosis, the role of PERK/eIF2 alpha/ATF4 integrated stress response (ISR) in astrocyte apoptosis mediated by oxygen-glucose-serum deprivation (OGSD)/restoration remains uncertain. Astrocytes were subjected to a combination of oxygen, glucose, and serum deprivation for 8h followed by restoration. Hoechst 33342 staining was performed to quantify apoptotic astrocytes and cell viability was assessed with Cell Counting Kit-8 (CCK8). Immunocytochemical analysis and Western blotting for some related molecules, including pancreatic ER stress kinase (PERK), p-PERK, eukaryotic initiation factor 2 alpha (eIF2 alpha), p-eIF2 alpha, activating transcription factor 4 (ATF4), caspase-12, were examined. Caspase activation and apoptosis were detected in neonatal rat astrocytes from spinal cord subjected to OGSD/restoration. We also observed an increase in cytoplasmic staining of p-eIF2 alpha in astrocytes (8h OGSD/15 min restoration) compared with that of non-treated cells. In addition, we found the sequential activation of PERK, eIF2 alpha, and ATF4 during OGSD/restoration by Western blotting. These results indicate that both the PERK/eIF2 alpha/ATF4 ISR and activation of caspase-12 may be involved in apoptosis of spinal cord astrocytes induced by OGSD/restoration. |
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Authors:
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Ailiang Zhang; Jie Zhang; Peng Sun; Changjiang Yao; Changhui Su; Tao Sui; Hua Huang; Xiaojian Cao; Yingbin Ge |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-02 |
Journal Detail:
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Title: Neuroscience letters Volume: 478 ISSN: 1872-7972 ISO Abbreviation: Neurosci. Lett. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-07 Completed Date: 2010-08-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 32-6 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Activating Transcription Factor 4
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physiology Animals Animals, Newborn Apoptosis* Astrocytes / cytology*, metabolism Caspase 12 / physiology* Cell Survival Cells, Cultured Culture Media, Serum-Free Enzyme Activation Eukaryotic Initiation Factor-2 / physiology* Glucose / deficiency* Oxygen / metabolism* Rats Rats, Sprague-Dawley Spinal Cord / cytology*, metabolism eIF-2 Kinase / physiology |
| Chemical | |
Reg. No./Substance:
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0/Atf4 protein, rat; 0/Casp12 protein, rat; 0/Culture Media, Serum-Free; 0/Eukaryotic Initiation Factor-2; 145891-90-3/Activating Transcription Factor 4; 50-99-7/Glucose; 7782-44-7/Oxygen; EC 2.7.10.-/PERK kinase; EC 2.7.11.1/eIF-2 Kinase; EC 3.4.22.-/Caspase 12 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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