Document Detail


EGR4 displays both a cell- and intracellular-specific localization pattern in the developing murine testis.
MedLine Citation:
PMID:  20925118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spermatogenesis is an intricately regulated process of cellular differentiation transforming spermatogonial stem cells to spermatozoa. Elimination of the transcription factor EGR4 generates subfertile male mice yet the expression and function of EGR4 in the mammalian testis has yet to be fully investigated. We performed in situ hybridization and immunofluorescence to identify Egr4 transcript and protein localization in the developing murine testis. EGR4 was detected in both germ and somatic cells in the neonatal testis but was specific to germ cells inside the seminiferous epithelium from juvenile development onward. EGR4 also displayed distinct intracellular localization patterns within specific cell populations of the testis. In addition, Egr4-deficient testis tubules regress from relatively normal to Sertoli cell and undifferentiated spermatogonia only over time. Taken together, these data suggest that Egr4 may regulate spermatogenesis at multiple steps, with roles in the dividing Sertoli cells, peritubular myoid cells, and the meiotic and elongating haploid germ cell populations.
Authors:
Cathryn A Hogarth; Debra Mitchell; Christopher Small; Michael Griswold
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Developmental dynamics : an official publication of the American Association of Anatomists     Volume:  239     ISSN:  1097-0177     ISO Abbreviation:  Dev. Dyn.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-27     Completed Date:  2011-02-03     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  9201927     Medline TA:  Dev Dyn     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3106-14     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley-Liss, Inc.
Affiliation:
School of Molecular Biosciences and Centre for Reproductive Biology, Washington State University, Pullman, Washington 99164, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Early Growth Response Transcription Factors / genetics,  metabolism*
Fluorescent Antibody Technique
Gene Expression Regulation, Developmental / genetics,  physiology
In Situ Hybridization
Male
Mice
Reverse Transcriptase Polymerase Chain Reaction
Spermatogenesis / genetics,  physiology
Testis / metabolism*
Grant Support
ID/Acronym/Agency:
HD R01 10808/HD/NICHD NIH HHS; R01 HD010808-32/HD/NICHD NIH HHS; U54 42454//PHS HHS; U54 HD042454-06/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Early Growth Response Transcription Factors; 0/Egr4 protein, mouse
Comments/Corrections

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