Document Detail


The EGFR is required for proper innervation to the skin.
MedLine Citation:
PMID:  18830272     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
EGFR family members are essential for proper peripheral nervous system development. A role for EGFR itself in peripheral nervous system development in vivo, however, has not been reported. We investigated whether EGFR is required for cutaneous innervation using Egfr null and skin-targeted Egfr mutant mice. Neuronal markers; including PGP9.5, GAP-43, acetylated tubulin, and neurofilaments; revealed that Egfr null dorsal skin was hyperinnervated with a disorganized pattern of innervation. In addition, receptor subtypes such as lanceolate endings were disorganized and immature. To determine whether the hyperinnervation phenotype resulted from a target-derived effect of loss of EGFR, mice lacking EGFR expression in the cutaneous epithelium were examined. These mice retained other aspects of the cutaneous Egfr null phenotype but exhibited normal innervation. The sensory deficits in Egfr null dorsal skin were not associated with any abnormality in the morphology or density of dorsal root ganglion (DRG) neurons or Schwann cells. However, explant and dissociated cell cultures of DRG revealed more extensive branching in Egfr null cultures. These data demonstrate that EGFR is required for proper cutaneous innervation during development and suggest that it limits axonal outgrowth and branching in a DRG-autonomous manner.
Authors:
Adel Maklad; Jodi R Nicolai; Kyle J Bichsel; Jackie E Evenson; Tang-Cheng Lee; David W Threadgill; Laura A Hansen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2008-10-02
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  129     ISSN:  1523-1747     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-02-11     Completed Date:  2009-03-11     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  690-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
GAP-43 Protein / metabolism
Ganglia, Spinal / metabolism
Mice
Mice, Mutant Strains
Mice, Transgenic
Models, Biological
Mutation
Neurites / metabolism*
Neurons / metabolism
Receptor, Epidermal Growth Factor / metabolism*,  physiology
Schwann Cells / metabolism
Skin / innervation*
Tubulin / metabolism
Ubiquitin Thiolesterase / metabolism
Grant Support
ID/Acronym/Agency:
1 C06 RR17417-01/RR/NCRR NIH HHS; P20 RR-02-003/RR/NCRR NIH HHS; P20 RR018788/RR/NCRR NIH HHS; P20 RR018788-010001/RR/NCRR NIH HHS; R01 ES015585/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/GAP-43 Protein; 0/Tubulin; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 3.1.2.-/PGP9.5 protein, mouse; EC 3.1.2.15/Ubiquitin Thiolesterase
Comments/Corrections
Comment In:
J Invest Dermatol. 2009 Mar;129(3):524   [PMID:  19209150 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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