Document Detail


EGFR regulates the side population in head and neck squamous cell carcinoma.
MedLine Citation:
PMID:  16540898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To identify the presence of side population (SP) cells in established head and neck squamous carcinoma cell (HNSCC) lines and to determine the role of EGFR in the regulation of the side population of these cells.
METHODS: SP cells were identified using flow cytometry analysis by the ability of these cells to extrude the Hoechst 33342 dye via the drug transporter BCRP1/ABCG2. Effect of EGFR on the side population was determined also by difference in Hoechst extrusion and by immunofluorescence. Immunohistochemical staining was performed to show the presence of the BCRP1/ABCG2 transporter and the phosphorylated form of EGFR in HNSCC tissue.
RESULTS: SP cells are present in HNSCC cell lines. With the Hoechst 33342 extrusion assay, SP cells were found to comprise an average of 0.69% of the UMSCC10B cells and 0.91% of HN12 cells. Addition of the EGF ligand increased the SP population while inactivation of the EGFR kinase by Iressa significantly decreased SP.
CONCLUSION: In established head and neck squamous cell carcinoma cell lines, SP cells were found using methods that determine expression and function of the drug transporter BCRP1/ABCG2. Activation of EGFR, a gene implicated in tumorigenesis in HNSCC leads to increased SP, and conversely, inhibition of EGFR leads to decrease in SP. This finding could help explain the role of EGFR in regulating cancer stem cells and thus tumorigenesis in HNSCC.
Authors:
Jocelyn S Chen; Francisco S Pardo; Jessica Wang-Rodriguez; Theresa S Chu; Jay Patrick Lopez; Joseph Aguilera; Xabier Altuna; Robert A Weisman; Weg M Ongkeko
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Laryngoscope     Volume:  116     ISSN:  0023-852X     ISO Abbreviation:  Laryngoscope     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-16     Completed Date:  2006-04-06     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8607378     Medline TA:  Laryngoscope     Country:  United States    
Other Details:
Languages:  eng     Pagination:  401-6     Citation Subset:  IM    
Affiliation:
Division of Head and Neck Surgery, Department of Surgery, University of California, San Diego, San Diego, California 92093, USA.
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MeSH Terms
Descriptor/Qualifier:
ATP-Binding Cassette Transporters / metabolism
Antineoplastic Agents / pharmacology
Carcinoma, Squamous Cell / drug therapy,  metabolism*,  pathology
Cell Line, Tumor
Cell Proliferation
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression Regulation, Neoplastic / drug effects,  physiology*
Head and Neck Neoplasms / drug therapy,  metabolism*,  pathology
Humans
Immunohistochemistry
Neoplasm Proteins / metabolism
Quinazolines / pharmacology
Receptor, Epidermal Growth Factor / antagonists & inhibitors,  biosynthesis*
Chemical
Reg. No./Substance:
0/ABCG2 protein, human; 0/Antineoplastic Agents; 0/Neoplasm Proteins; 0/Quinazolines; EC 2.7.10.1/Receptor, Epidermal Growth Factor; S65743JHBS/gefitinib

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